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Table 3 Proteins with differential abundances in the ethanol producer compared to WT of Synechococcus 7002 at day 8 of the cultivation

From: Systems analysis of ethanol production in the genetically engineered cyanobacterium Synechococcus sp. PCC 7002

ID cyano-base Protein ID NCBI Protein name Gene name Function (putative) Fold change compared to WT (day 8)
Producer (day 8) Producer (day 22) WT (day 22)
Proteins with lower abundance in the producer strain compared to WT
 A0250 ACA98261 PEP synthase ppsA Glycolysis 0.77 (±0.24) 0.97 (±0.54) 2.75 (±0.21)
 A0115 ACA98130 Glycosyl hydrolase   Sugar metabolism 0.02 (±0.028) 0.01 (±0.02) 1.49 (±0.13)
 A1150 ACA99150 Glucosamine-fructose-6-phosphatase glmS Sugar metabolism 0.32 (±0.12) 0.16 (±0.06) 1.05 (±0.15)
 A1172 ACA99171 1-Deoxy-D-xylulose-5-phosphate synthase dxs Isoprenoid synthesis 0.45 (±0.12) 0.53 (±0.17) 1.06 (±0.14)
 A2851 ACB00819 GG-phosphate synthase ggpS Osmolyte synthesis 0.52 (±0.08) 0.09 (±0.02) 0.42 (±0.05)
 A0330 ACA98340 4-Alpha-glucotransferase malQ Starch metabolism 0.18 (±0.07) 0.46 (±0.08) 1.26 (±0.16)
 A2069 ACB00056 Adenosylhomocysteinase ahcY Amino acid synthesis 0.45 (±0.01) 0.47 (±0.06) 0.73 (±0.06)
 A2819 ACB00788 Glycosyl hydrolase   Sugar metabolism 0.02 (±0.01) 0.07 (±0.08) 0.84 (±0.17)
 A2665 ACB00642 Phosphoribulokinase prk Calvin cycle 0.33 (±0.12) 0.05 (±0.02) 0.66 9 (±0.26)
 A0106 ACA98121 Glyceraldehyde-3-phosphate dehydrogenase gapI Calvin cycle 0.48 (±0.02) 0.46 (±0.04) 0.83 (±0.04)
 A0353 ACA98363 Pyruvate dehydrogenase I   Pyruvate to acetylCoA 0.53 (±0.05) 0.35 (±0.03) 1.00 (±0.03)
 A0452 ACA98460 Aldose 1-epimerase   Gluconeogenesis 0.33 (±0.13) 0.23 (±0.07) 1.43 (±0.09)
 A1285 ACA99282 PII protein glnB Sensing cellular N 0.38 (±0.09) 0.29 (±0.09) 1.04 (±0.17)
 A1549 ACA99540 Glycine dehydrogenase gcvP Glycine cleavage system P-protein 0.89 (±0.90) 0.42 (±0.18) 2.36 (±0.23)
Proteins with higher abundance in producer strain compared to WT
 A1321 ACA99318 Flavoprotein flv3 Oxygen reducing flavodiiron protein 5.64 (±0.9) 6.33 (±0.14) 1.86 (±0.49)
 A0605 ACA98612 Glycerol dehydrogenase gldA Glycerol metabolism 3.54 (±0.98) 14.94 (±5.50) 4.69 (±1.50)
 A2458 ACB00436 Chaperonin 60 kDa groEL Heat shock protein 1.70 (±0.05) 1.10 (±0.29) 0.98 (±0.10)
 A1803 ACA99791 Carbon concentrating mechanism protein ccmK 2.33 (±0.30) 1.61 (±0.40) 1.1 (±0.10)
 A2457 ACB00435 Chaperonin 10 kDa groES Heat shock protein 330.90 (±176.00) 219.13 (±270.00) 13.68 (±21.50)
 A0147 ACA98161 Chaperonin Cpn60 groEL II Heat shock protein 5.96 (±1.55) 3.08 (±1.20) 3.56 (±0.60)
 A1800 ACA99788 Carbon concentrating mechanism protein ccmM 1.73 (±0.27) 0.38 (±0.40) 0.16 (±0.30)
   Pyruvate decarboxylase pdc Present Present Absent
  1. The fold changes to WT values on day 8 are given as fold change values ± standard deviation. Data present mean values and standard deviation of two independent cultures with two technical repeats each