Fig. 4
From: Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date
Catalytic efficiency on different substrates during peroxidase evolution. Changes of the relative catalytic efficiencies (k cat /K m) on ABTS, DMP, Mn2+, RB5, and VA (the two former at high and low efficiency oxidation sites) during evolution, with the maximum for each substrate taken as 100%. The CaPo-bis and CaPo-tris alternative ancestors showed the following relative catalytic efficiencies: (i) ABTS (low efficiency), 100 and 94%, respectively; (ii) DMP (low efficiency), 4.5 and 6.0%, respectively; (iii) Mn2+, 0.5% for CaPo-tris (no activity detected with CaPo-bis); and (iv) no activity on ABTS (high efficiency), DMP (high efficiency), RB5, and VA