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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date

Fig. 5

pH stability of the resurrected ancestors and extant LiPH8. a Residual activities were measured with 2.5 mM ABTS at optimal pH (see Table 1) after 4 h incubation at different pH values (25 °C) and referred to activity after 1 min incubation of each of them at pH 5. The CaPo-bis and CaPo-tris alternative ancestors showed the following relative residual activities: (i) pH 2, 0%; (ii) pH 3, 10 and 44%, respectively; (iii) pH 4, 88 and 89%, respectively; (iv) pH 5, 85 and 90%, respectively; (v) pH 6, 93 and 97%, respectively; (vi) pH 7, 93 and 95%, respectively; (vii) pH 8, 84 and 95%, respectively; (viii) pH 9, 88 and 28%, respectively; and (ix) pH 10, 0%. b Comparison of the VA catalytic efficiency (s−1 mM−1; dashed line) and enzyme stability at pH 3 (continuous line) vs evolutionary distance [31]. The pH 3 residual activities were measured as indicated in a. Means and 95% confidence limits

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