From: Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date
CaPo | CaCD | AVP | ALiP | LiPH8 | |
---|---|---|---|---|---|
Metal ion | |||||
Mn2+ | |||||
K m | 700 ± 48 | 275 ± 41 | 62 ± 10 | –a | – |
k cat | 185 ± 3 | 170 ± 6 | 106 ± 4 | – | – |
k cat /K m | 260 ± 15 | 617 ± 80 | 1710 ± 240 | – | – |
Aromatics | |||||
DMPb (low efficiency) | |||||
K m | 32,900 ± 2700 | 66,800 ± 3800 | 32,500 ± 12,100 | – | – |
k cat | 221 ± 9 | 109 ± 4 | 31 ± 5 | – | – |
k cat /K m | 6.7 ± 0.3 | 1.6 ± 0.03 | 1.0 ± 0.2 | – | – |
DMP (high efficiency) | |||||
K m | – | – | 5.3 ± 1.1 | 34.0 ± 5.4 | 4.0 ± 0.07 |
k cat | – | – | 4.5 ± 0.1 | 18.3 ± 0.7 | 6.9 ± 0.5 |
k cat /K m | – | – | 837 ± 162 | 537 ± 55 | 600 ± 36 |
VA | |||||
K m | – | – | 299 ± 104 | 773 ± 155 | 79.3 ± 18 |
k cat | – | – | 7.1 ± 0.5 | 21.3 ± 1.0 | 16.2 ± 0.8 |
k cat /K m | – | – | 24 ± 7 | 28 ± 5 | 205 ± 4 |
Dyes | |||||
ABTSb (low efficiency) | |||||
K m | 3170 ± 270 | 1280 ± 350 | 2150 ± 420 | – | – |
k cat | 539 ± 24 | 103 ± 10 | 25 ± 2 | – | – |
k cat /K m | 170 ± 8 | 80 ± 15 | 12 ± 1 | – | – |
ABTS (high efficiency) | |||||
K m | – | – | 5.4 ± 0.7 | 13.7 ± 3.5 | 21.3 ± 2.3 |
k cat | – | – | 2.1 ± 0.1 | 12.5 ± 0.7 | 6.5 ± 0.2 |
k cat /K m | – | – | 400 ± 44 | 911 ± 212 | 300 ± 25 |
RB5 | |||||
K m | – | – | 4.8 ± 0.8 | 12.6 ± 3.6 | – |
k cat | – | – | 2.4 ± 0.2 | 5.4 ± 0.8 | – |
k cat /K m | – | – | 504 ± 50 | 428 ± 68 | – |
Direction of evolution |
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