Fig. 1From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistanceMaps of shuttle vectors for BdhA expression in C. thermocellum. The bdhA gene from T. pseudethanolicus 39E (Teth39_1597) was expressed under the control of the S-layer (a), Clo1313_1809 (b), and enolase (c) promoters. Shuttle vectors contain a C-terminal 6X His-tag version of bdhA, a Rho-independent terminator, the pyrF (from C. bescii) cassette for selection of plasmid transformants, and pBAS2 sequences for replication in C. thermocellum Back to article page