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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance

Fig. 1

Maps of shuttle vectors for BdhA expression in C. thermocellum. The bdhA gene from T. pseudethanolicus 39E (Teth39_1597) was expressed under the control of the S-layer (a), Clo1313_1809 (b), and enolase (c) promoters. Shuttle vectors contain a C-terminal 6X His-tag version of bdhA, a Rho-independent terminator, the pyrF (from C. bescii) cassette for selection of plasmid transformants, and pBAS2 sequences for replication in C. thermocellum

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