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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance

Fig. 4

Comparison of fermentation products and in vitro reduction activity of furan derivatives by JWCT02, JWCT06, and JWCT08 strains. a, b Cellobiose consumed and fermentation products of C. thermocellum. JWCT02, JWCT06, and JWCT08 strains were grown in defined medium with 5 g/L cellobiose containing 10 mM furfural (a) or HMF (b). Results are the mean of duplicate experiments and error bars indicate s.d. c, d In vitro assays of reduction activity of furfural (c) or HMF (d). Crude protein extracts of JWCT02, JWCT06, and JWCT08 strains were assayed for reduction activity using NAD(P)H as cofactor. JWCT02, the parent control strain; JWCT06, containing PS-layer -bdhA; JWCT08, containing Penolase -bdhA. Results are the mean of triplicate experiments and error bars indicate s.d

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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