Fig. 3

Engineering of acetyl-TAG production in a wild S. cerevisiae strain. A haploid version of the xylose-metabolizing GLBRCY2A strain was engineered for reduced lcTAG production. The schematic diagram in the upper subpanel of (a) indicates the predicted sizes (in kb) of PCR products generated by primers specific for wild-type ARE1, ARE2, DGA1, and LRO1 or their corresponding marker-rescued deletions. Actual PCRs from the Y40 (WT) or Y40 strain containing all four deletions (4KO) are indicated alongside flanking DNA standards in the lower subpanel. PCR-verification of EaDAcT insertion into the genome of 4KO is shown in (b). A schematic diagram showing predicted products from the primers used is on the left. Actual PCR confirmation in the indicated strains is shown on the right