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Table 1 Fermentation properties for engineered and evolved S. cerevisiae strains

From: Metabolic engineering of Saccharomyces cerevisiae to produce a reduced viscosity oil from lignocellulose

Medium Aerobic YPDX Aerobic ACSH
Strain SCY62+EaDAcT H1246+EaDAcT 4KO+EaDAcT SCY62+EaDAcT H1246+EaDAcT 4KO+EaDAcT
Acetyl-TAG titera 2400 ± 65 1500 ± 180 3000 ± 42 2200 ± 95 160 ± 19 1100 ± 6.2
Volumetric acetyl-TAG titerb 15 ± 2.0 6.9 ± 0.11 32 ± 2.5 14 ± 0.13 0.30 ± 0.036 7.9 ± 0.087
lcTAG titerc 6800 ± 0.20 ND 4200 ± 44 6300 ± 0.10 96 ± 1.1 2300 ± 2.2
Volumetric lcTAG titerd 43 ± 5.9 ND 45 ± 3.3 4.0 ± 0.37 0.18 ± 0.016 16 ± 0.10
% acetyl-TAGse 31 ± 0.21 100 ± 0 48 ± 0.52 31 ± 0.097 67 ± 0.99 38 ± 0.36
Estimated growth ratef 0.34 ± 0.049 0.25 ± 0.035 0.58 ± 0.044 0.021 ± 0.0004 0.074 ± 0.002 0.29 ± 0.0003
Y gacTAG/glc 260 ± 9.5 130 ± 9.8 510 ± 48 120 ± 18 8.0 ± 1.5 120 ± 0.67
Y hlcTAG/glc 750 ± 32 ND 710 ± 65 330 ± 51 4.8 ± 0.76 250 ± 2.6
  1. ND not detected
  2. aIn μg of acetyl-TAG/g of dry cell weight (DCW)
  3. bIn mg of acetyl-TAG/L
  4. cIn μg of lcTAG/g of DCW
  5. dIn mg of lcTAG/L
  6. eMole percentage of acetyl-TAG produced out of the total TAG produced
  7. fIn g of DCW/L/h within 18 h (in YPDX medium) or 24 h (in ACSH medium) of growth
  8. gYield in μg of acetyl-TAG produced/g of glucose consumed
  9. hYield in μg lcTAG produced/g of glucose consumed