TY - JOUR AU - Gunawan, Christa AU - Xue, Saisi AU - Pattathil, Sivakumar AU - da Costa Sousa, Leonardo AU - Dale, Bruce E. AU - Balan, Venkatesh PY - 2017 DA - 2017/03/29 TI - Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover JO - Biotechnology for Biofuels SP - 82 VL - 10 IS - 1 AB - Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass). Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues. The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery. Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins. Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans. SN - 1754-6834 UR - https://doi.org/10.1186/s13068-017-0757-5 DO - 10.1186/s13068-017-0757-5 ID - Gunawan2017 ER -