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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus

Fig. 3

Cell wall composition and gene expression analyses in OXPdDUF266A transgenic lines. a Gene construct used for generating Populus transgenic plants overexpressing PdDUF266A. b RT-PCR analysis of PdDUF266A expression in two independent Populus transgenic lines overexpressing PdDUF266A. Total RNA was extracted from petiole of mature leaf. PdUBCc was used as an internal control. PCR cycle numbers are indicated. Transgenic lines (OXPdDUF266A-1 and OXPdDUF266A-2) and WV94 (wild type) were grown under greenhouse conditions. c Sugar content analysis by means of ion chromatography after two-step acid treatment. d Total lignin content by measuring acid soluble/insoluble separation. e Cellulose content analysis by means of Anthrone dye staining. f qRT-PCR analysis of cellulose biosynthesis-related genes in mature leaf and xylem. PdUBCc was used as an internal control to normalize the qRT-PCR result. Shown are mean values of three technical repeats from two biological repeats for each transgenic line ± S.D. Asterisks indicate statistical significance (p < 0.01)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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