Fig. 1

Schematic representation of the two main experiments. Three different temperatures were assayed. When working with wild isolates, we set the temperature of fermentation to room temperature (24 °C) or, for screening, the minimum allowed by the Tecan plate reader (28 °C). Adaptive evolution experiments and comparisons of the synthetic hybrids to Y73 were run at 30 °C, the temperature previously used to experimentally evolve Y73 [24]. G generations