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Table 2 Strains, plasmids, and primers used in this study

From: Enhanced fermentative performance under stresses of multiple lignocellulose-derived inhibitors by overexpression of a typical 2-Cys peroxiredoxin from Kluyveromyces marxianus

Strains Genotype Source
Y179 K. marxianus, wild-type Lab stock
280 S. cerevisiae, MAT a, his3-1, leu2-0, met 15-0, ura3 Lab stock
DH5α E. coli, for genetic manipulation Lab stock
TPX1 S. cerevisiae overexpressing KmTPX1 gene, transformed from 280 This study
423 S. cerevisiae with pRS423 plasmid, transformed from 280 This study
Plasmids Characteristic Source and reference
pRS423 Yeast episomal vector with HIS3 marker [37]
p423TPX KmTPX1 in pRS423 This study
Primers
 KmTPX1-F 5′-CTTgagctcAATGTCTCGTCTCGTCTCGT-3′
 KmTPX1-R 5′-TCCccgcggGGCTAAGCCAATAACTTATT-3′
 RT-TPX1-F 5′-CTCAAGTTTTGTTCGCTTCCAC-3′
 RT-TPX2-R 5′-AAGTCGTTGATGGTGATGTGTCT-3′
 ACT1-F 5′-ACGTTGTTCCAATCTACGCC-3′
 ACT1-R 5′-CTTGTTCGAAGTCCAAAGCG-3′
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