Table 2 Strains, plasmids, and primers used in this study
Strains | Genotype | Source |
|---|---|---|
Y179 | K. marxianus, wild-type | Lab stock |
280 | S. cerevisiae, MAT a, his3-1, leu2-0, met 15-0, ura3 | Lab stock |
DH5α | E. coli, for genetic manipulation | Lab stock |
TPX1 | S. cerevisiae overexpressing KmTPX1 gene, transformed from 280 | This study |
423 | S. cerevisiae with pRS423 plasmid, transformed from 280 | This study |
Plasmids | Characteristic | Source and reference |
|---|---|---|
pRS423 | Yeast episomal vector with HIS3 marker | [37] |
p423TPX | KmTPX1 in pRS423 | This study |
Primers | ||
 KmTPX1-F | 5′-CTTgagctcAATGTCTCGTCTCGTCTCGT-3′ | |
 KmTPX1-R | 5′-TCCccgcggGGCTAAGCCAATAACTTATT-3′ | |
 RT-TPX1-F | 5′-CTCAAGTTTTGTTCGCTTCCAC-3′ | |
 RT-TPX2-R | 5′-AAGTCGTTGATGGTGATGTGTCT-3′ | |
 ACT1-F | 5′-ACGTTGTTCCAATCTACGCC-3′ | |
 ACT1-R | 5′-CTTGTTCGAAGTCCAAAGCG-3′ | |