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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Co-production of hydrogen and ethanol from glucose in Escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd)

Fig. 3

Effect of inducer concentration on co-production of H2 and ethanol by SH5Δpgi_ZG. a SDS-PAGE analysis of Zwf (55 kDa) and Gnd (51 kDa) in soluble fraction. b Enzyme activity of Zwf and Gnd of SH5Δpgi_ZG induced with different IPTG concentrations. c Metabolite yields and ratios of CO2 to H2 evolution of SH5Δpgi_ZG induced with 0, 0.02, 0.05, 0.1, 0.2, and 0.3 mM IPTG

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