Co-production of hydrogen and ethanol from glucose in Escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd)

Table 2 Co-production by recombinant SH5Δpgi strains overexpressing Zwf and Gnd

Substrate	Strains	Yields of metabolites (mol mol⁻¹)
Substrate	Strains	H₂	Ethanol	Acetate
Glucose	SH5	1.44 ± 0.07	0.79 ± 0.02	0.67 ± 0.04
	SH5Δpgi_Z	1.81 ± 0.08	0.90 ± 0.02	0.86 ± 0.03
	SH5Δpgi_ZG	1.68 ± 0.06	1.44 ± 0.03	0.22 ± 0.02
Gluconate	SH5	1.72 ± 0.09	0.58 ± 0.01	1.31 ± 0.03
	SH5Δpgi_Z	1.70 ± 0.05	0.68 ± 0.01	1.25 ± 0.04
	SH5Δpgi_ZG	1.69 ± 0.07	0.92 ± 0.02	0.85 ± 0.02

ISSN: 2731-3654