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Table 3 Relative transcription levels of key glycolytic enzymes in SH5Δpgi_Z and SH5Δpgi_ZG

From: Co-production of hydrogen and ethanol from glucose in Escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd)

Gene

SH5Δpgi_Z (0.1 mM)

SH5Δpgi_ZG (0.1 mM)

SH5Δpgi_ZG (0.2 mM)

pgi

0.00

0.00

0.00

zwf

2090.91 ± 146.36

2693.55 ± 212.52

8001.77 ± 400.09

gnd

6.03 ± 0.21

2155.47 ± 73.18

6039.95 ± 259.79

pfkA

2.16 ± 0.11

3.59 ± 0.06

5.48 ± 0.13

pfkB

2.59 ± 0.13

1.29 ± 0.03

1.19 ± 0.03

gapA

34.12 ± 1.54

44.01 ± 0.96

80.95 ± 3.24

edd

1.41 ± 0.02

1.09 ± 0.01

0.87 ± 0.01

tktA

12.20 ± 0.24

5.72 ± 0.12

7.28 ± 0.16

pflB

42.99 ± 1.38

21.51 ± 0.65

37.04 ± 0.74

fhlA

0.38 ± 0.01

0.52 ± 0.01

0.43 ± 0.01

udhA

0.45 ± 0.01

0.55 ± 0.01

0.28 ± 0.01

adhE

4.51 ± 0.05

7.22 ± 0.12

13.76 ± 0.47

  1. The result was from three individual experiment repeats
  2. rpoD was used as the endogenous control and the transcriptional level of rpoD was considered as 1