Skip to main content

Table 2 Specific activities in whole-cells and broken-cell extracts

From: Citrobacter amalonaticus Y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery

Cella

Whole-cells CO-dependent H2 production (mmol g−1 h−1)

Formate-dependent H2 production (mmol g−1 h−1)

Crude-cell lysates CODH (μmol min−1 mg−1)

Evolving hydrogenase (μmol min−1 mg−1)

Uptake hydrogenase (μmol min−1 mg−1)

Cultivated on maltose

 Y19 WT (no CO)

ND

16.2 ± 0.7

ND

1.6 ± 0.1

9.8 ± 0.5

 Y19 WT (20% CO)

22.1 ± 1.1

12.0 ± 0.6

32.1 ± 6.1

2.5 ± 0.2

7.9 ± 0.4

 Y19-PR1 (no CO)

23.8 ± 1.2

15.2 ± 0.7

49.3 ± 6.7

2.8 ± 0.2

8.9 ± 0.5

 Y19-PR1 (20% CO)

22.9 ± 1.2

10.7 ± 0.5

45.6 ± 6.7

2.3 ± 0.1

8.2 ± 0.4

Cultivated on glucose

 Y19 WT (no CO)

ND

18.2 ± 0.8

ND

1.9 ± 0.2

12.4 ± 0.7

 Y19 WT (20% CO)

3.7 ± 0.2

14.0 ± 0.7

2.1 ± 0.1

3.2 ± 0.1

9.1 ± 0.4

 Y19-PR1 (no CO)

19.8 ± 0.9

17.2 ± 0.9

36.3 ± 1.8

3.6 ± 0.2

11.9 ± 0.6

 Y19-PR1 (20% CO)

20.7 ± 1.1

15.3 ± 0.7

41.9 ± 2.7

3.3 ± 0.2

8.9 ± 0.4

  1. CODH CO dehydrogenase, CO-hyd CO-dependent hydrogenase, ND not detected
  2. aCells were grown in either presence (20%, v/v) or absence of CO in the head space