|
Cella
|
Whole-cells CO-dependent H2 production (mmol g−1 h−1)
|
Formate-dependent H2 production (mmol g−1 h−1)
|
Crude-cell lysates CODH (μmol min−1 mg−1)
|
Evolving hydrogenase (μmol min−1 mg−1)
|
Uptake hydrogenase (μmol min−1 mg−1)
|
|---|
|
Cultivated on maltose
|
|
Y19 WT (no CO)
|
ND
|
16.2 ± 0.7
|
ND
|
1.6 ± 0.1
|
9.8 ± 0.5
|
|
Y19 WT (20% CO)
|
22.1 ± 1.1
|
12.0 ± 0.6
|
32.1 ± 6.1
|
2.5 ± 0.2
|
7.9 ± 0.4
|
|
Y19-PR1 (no CO)
|
23.8 ± 1.2
|
15.2 ± 0.7
|
49.3 ± 6.7
|
2.8 ± 0.2
|
8.9 ± 0.5
|
|
Y19-PR1 (20% CO)
|
22.9 ± 1.2
|
10.7 ± 0.5
|
45.6 ± 6.7
|
2.3 ± 0.1
|
8.2 ± 0.4
|
|
Cultivated on glucose
|
|
Y19 WT (no CO)
|
ND
|
18.2 ± 0.8
|
ND
|
1.9 ± 0.2
|
12.4 ± 0.7
|
|
Y19 WT (20% CO)
|
3.7 ± 0.2
|
14.0 ± 0.7
|
2.1 ± 0.1
|
3.2 ± 0.1
|
9.1 ± 0.4
|
|
Y19-PR1 (no CO)
|
19.8 ± 0.9
|
17.2 ± 0.9
|
36.3 ± 1.8
|
3.6 ± 0.2
|
11.9 ± 0.6
|
|
Y19-PR1 (20% CO)
|
20.7 ± 1.1
|
15.3 ± 0.7
|
41.9 ± 2.7
|
3.3 ± 0.2
|
8.9 ± 0.4
|
-
CODH CO dehydrogenase, CO-hyd CO-dependent hydrogenase, ND not detected
-
aCells were grown in either presence (20%, v/v) or absence of CO in the head space
