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Table 2 Specific activities in whole-cells and broken-cell extracts

From: Citrobacter amalonaticus Y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery

Cella Whole-cells CO-dependent H2 production (mmol g−1 h−1) Formate-dependent H2 production (mmol g−1 h−1) Crude-cell lysates CODH (μmol min−1 mg−1) Evolving hydrogenase (μmol min−1 mg−1) Uptake hydrogenase (μmol min−1 mg−1)
Cultivated on maltose
 Y19 WT (no CO) ND 16.2 ± 0.7 ND 1.6 ± 0.1 9.8 ± 0.5
 Y19 WT (20% CO) 22.1 ± 1.1 12.0 ± 0.6 32.1 ± 6.1 2.5 ± 0.2 7.9 ± 0.4
 Y19-PR1 (no CO) 23.8 ± 1.2 15.2 ± 0.7 49.3 ± 6.7 2.8 ± 0.2 8.9 ± 0.5
 Y19-PR1 (20% CO) 22.9 ± 1.2 10.7 ± 0.5 45.6 ± 6.7 2.3 ± 0.1 8.2 ± 0.4
Cultivated on glucose
 Y19 WT (no CO) ND 18.2 ± 0.8 ND 1.9 ± 0.2 12.4 ± 0.7
 Y19 WT (20% CO) 3.7 ± 0.2 14.0 ± 0.7 2.1 ± 0.1 3.2 ± 0.1 9.1 ± 0.4
 Y19-PR1 (no CO) 19.8 ± 0.9 17.2 ± 0.9 36.3 ± 1.8 3.6 ± 0.2 11.9 ± 0.6
 Y19-PR1 (20% CO) 20.7 ± 1.1 15.3 ± 0.7 41.9 ± 2.7 3.3 ± 0.2 8.9 ± 0.4
  1. CODH CO dehydrogenase, CO-hyd CO-dependent hydrogenase, ND not detected
  2. aCells were grown in either presence (20%, v/v) or absence of CO in the head space