Citrobacter amalonaticus Y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery

Table 2 Specific activities in whole-cells and broken-cell extracts

Cell^a	Whole-cells CO-dependent H₂ production (mmol g⁻¹ h⁻¹)	Formate-dependent H₂ production (mmol g⁻¹ h⁻¹)	Crude-cell lysates CODH (μmol min⁻¹ mg⁻¹)	Evolving hydrogenase (μmol min⁻¹ mg⁻¹)	Uptake hydrogenase (μmol min⁻¹ mg⁻¹)
Cultivated on maltose
Y19 WT (no CO)	ND	16.2 ± 0.7	ND	1.6 ± 0.1	9.8 ± 0.5
Y19 WT (20% CO)	22.1 ± 1.1	12.0 ± 0.6	32.1 ± 6.1	2.5 ± 0.2	7.9 ± 0.4
Y19-PR1 (no CO)	23.8 ± 1.2	15.2 ± 0.7	49.3 ± 6.7	2.8 ± 0.2	8.9 ± 0.5
Y19-PR1 (20% CO)	22.9 ± 1.2	10.7 ± 0.5	45.6 ± 6.7	2.3 ± 0.1	8.2 ± 0.4
Cultivated on glucose
Y19 WT (no CO)	ND	18.2 ± 0.8	ND	1.9 ± 0.2	12.4 ± 0.7
Y19 WT (20% CO)	3.7 ± 0.2	14.0 ± 0.7	2.1 ± 0.1	3.2 ± 0.1	9.1 ± 0.4
Y19-PR1 (no CO)	19.8 ± 0.9	17.2 ± 0.9	36.3 ± 1.8	3.6 ± 0.2	11.9 ± 0.6
Y19-PR1 (20% CO)	20.7 ± 1.1	15.3 ± 0.7	41.9 ± 2.7	3.3 ± 0.2	8.9 ± 0.4

CODH CO dehydrogenase, CO-hyd CO-dependent hydrogenase, ND not detected
^aCells were grown in either presence (20%, v/v) or absence of CO in the head space

ISSN: 2731-3654