Table 1 Strains and plasmids used in this study
From: Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica
Strain | Genotype or plasmid | Source |
|---|---|---|
E. coli | ||
DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+), λ− | [33] |
DH5α | pAD, UAS1B16TEF promoter | This study |
DH5α | pAD-TKL1, YALI0E06479g | This study |
DH5α | pAD-TAL1, YALI0F15587g | This study |
DH5α | pAD-GDN1, YALI0B15598g | This study |
DH5α | pAD-ZWF1, YALI0E22649g | This study |
Dh5 α | phrGFP II-C | Agilent |
Dh5 α | pAD-hrGFP | This study |
Dh5 α | PTKL1-gfp | This study |
Dh5 α | PTAL1-gfp | This study |
Dh5 α | PGDN1-gfp | This study |
Dh5 α | PZWF1-gfp | This study |
Y. lipolytica | ||
AMM | MATA, MK1: ura3-302 | [28] |
MK1 | MATA, UV-mutant | [21] |
AMM pAD-TKL1 | MATA, MK1: ura3-302, overexpression YALI0E06479g | This study |
AMM pAD-TAL1 | MATA, MK1: ura3-302, overexpression YALI0F15587g | This study |
AMM pAD-GDN1 | MATA, MK1: ura3-302, overexpression YALI0B15598g | This study |
AMM pAD-ZWF1 | MATA, MK1: ura3-302, overexpression YALI0E22649g | This study |
AMM PTKL1-gfp | MATA, MK1: ura3-302, promoter TKL1 fused to hrgfp | This study |
AMM PTAL1-gfp | MATA, MK1: ura3-302, promoter TAL1 fused to hrgfp | This study |
AMM PGDN1-gfp | MATA, MK1: ura3-302, promoter GDN1 fused to hrgfp | This study |
AMM PZWF1-gfp | MATA, MK1: ura3-302, promoter ZWF1 fused to hrgfp | This study |