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Table 3 Side-metabolites of Y. lipolytica strains in shake-flask experiments in Erlenmeyer flasks

From: Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Time (h) Strain Arabitol (g/L) Mannitol (g/L) Citric acid (g/L) α-Ketoglutaric acid (g/L)
24 MK1 0 0 0 0
AMM pAD-GND1 0.20 ± 0.01 0.05 ± 0.01 1.13 ± 0.01 0
AMM pAD-ZWF1 0.24 ± 0.03 0.01 ± 0.01 0.78 ± 0.01 0
AMM pAD-TKL1 0 0 0.84 ± 0.01 0
AMM pAD-TAL1 0 1.12 ± 0.03 0 0
48 MK1 0.40 ± 0.05 0.32 ± 0.06 2.60 ± 0.13 0.55 ± 0.02
AMM pAD-GND1 0.93 ± 0.05 2.15 ± 0.02 9.94 ± 0.11 0.44 ± 0.05
AMM pAD-ZWF1 1.33 ± 0.10 1.99 ± 0.23 8.58 ± 1.33 0.53 ± 0.08
AMM pAD-TKL1 0.87 ± 0.10 1.52 ± 0.07 6.32 ± 2.18 0.77 ± 0.19
AMM pAD-TAL1 1.04 ± 0.07 1.12 ± 0.03 5.05 ± 1.27 0.41 ± 0.04
54 MK1 0.43 ± 0.13 0.39 ± 0.09 3.05 ± 0.01 0.66 ± 0.06
AMM pAD-GND1 1.12 ± 0.01 3.00 ± 0.09 11.50 ± 0.25 0.45 ± 0.20
AMM pAD-ZWF1 1.58 ± 0.10 2.82 ± 0.15 10.33 ± 1.51 0.68 ± 0.15
AMM pAD-TKL1 1.09 ± 0.01 2.48 ± 0.02 7.64 ± 2.51 1.11 ± 0.38
AMM pAD-TAL1 1.52 ± 0.11 2.11 ± 0.45 5.85 ± 1.05 0.66 ± 0.13
72 MK1 0.64 ± 0.01 0.85 ± 0.04 4.54 ± 0.14 0.75 ± 0.05
AMM pAD-GND1 1.32 ± 0.12 5.45 ± 0.22 16.24 ± 0.71 0.64 ± 0.30
AMM pAD-ZWF1 2.01 ± 0.20 5.07 ± 0.01 13.62 ± 3.31 0.84 ± 0.32
AMM pAD-TKL1 1.35 ± 0.05 4.41 ± 0.05 10.33 ± 2.82 1.60 ± 0.61
AMM pAD-TAL1 1.70 ± 0.16 3.11 ± 0.65 7.92 ± 0.69 0.76 ± 0.1
94 MK1 0.89 ± 0.04 1.71 ± 0.16 5.98 ± 0.10 0.79 ± 0.08
AMM pAD-GND1 1.79 ± 0.12 7.63 ± 0.48 18.54 ± 1.67 0
AMM pAD-ZWF1 2.61 ± 0.16 7.02 ± 0.38 15.32 ± 4.28 0
AMM pAD-TKL1 1.53 ± 0.39 6.66 ± 0.78 12.70 ± 2.18 0.31 ± 0.10
AMM pAD-TAL1 2.30 ± 0.13 6.23 ± 0.36 11.16 ± 1.41 1.14 ± 0.52