From: Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica
Time (h) | Strain | Arabitol (g/L) | Mannitol (g/L) | Citric acid (g/L) | α-Ketoglutaric acid (g/L) |
---|---|---|---|---|---|
24 | MK1 | 0 | 0 | 0 | 0 |
AMM pAD-GND1 | 0.20 ± 0.01 | 0.05 ± 0.01 | 1.13 ± 0.01 | 0 | |
AMM pAD-ZWF1 | 0.24 ± 0.03 | 0.01 ± 0.01 | 0.78 ± 0.01 | 0 | |
AMM pAD-TKL1 | 0 | 0 | 0.84 ± 0.01 | 0 | |
AMM pAD-TAL1 | 0 | 1.12 ± 0.03 | 0 | 0 | |
48 | MK1 | 0.40 ± 0.05 | 0.32 ± 0.06 | 2.60 ± 0.13 | 0.55 ± 0.02 |
AMM pAD-GND1 | 0.93 ± 0.05 | 2.15 ± 0.02 | 9.94 ± 0.11 | 0.44 ± 0.05 | |
AMM pAD-ZWF1 | 1.33 ± 0.10 | 1.99 ± 0.23 | 8.58 ± 1.33 | 0.53 ± 0.08 | |
AMM pAD-TKL1 | 0.87 ± 0.10 | 1.52 ± 0.07 | 6.32 ± 2.18 | 0.77 ± 0.19 | |
AMM pAD-TAL1 | 1.04 ± 0.07 | 1.12 ± 0.03 | 5.05 ± 1.27 | 0.41 ± 0.04 | |
54 | MK1 | 0.43 ± 0.13 | 0.39 ± 0.09 | 3.05 ± 0.01 | 0.66 ± 0.06 |
AMM pAD-GND1 | 1.12 ± 0.01 | 3.00 ± 0.09 | 11.50 ± 0.25 | 0.45 ± 0.20 | |
AMM pAD-ZWF1 | 1.58 ± 0.10 | 2.82 ± 0.15 | 10.33 ± 1.51 | 0.68 ± 0.15 | |
AMM pAD-TKL1 | 1.09 ± 0.01 | 2.48 ± 0.02 | 7.64 ± 2.51 | 1.11 ± 0.38 | |
AMM pAD-TAL1 | 1.52 ± 0.11 | 2.11 ± 0.45 | 5.85 ± 1.05 | 0.66 ± 0.13 | |
72 | MK1 | 0.64 ± 0.01 | 0.85 ± 0.04 | 4.54 ± 0.14 | 0.75 ± 0.05 |
AMM pAD-GND1 | 1.32 ± 0.12 | 5.45 ± 0.22 | 16.24 ± 0.71 | 0.64 ± 0.30 | |
AMM pAD-ZWF1 | 2.01 ± 0.20 | 5.07 ± 0.01 | 13.62 ± 3.31 | 0.84 ± 0.32 | |
AMM pAD-TKL1 | 1.35 ± 0.05 | 4.41 ± 0.05 | 10.33 ± 2.82 | 1.60 ± 0.61 | |
AMM pAD-TAL1 | 1.70 ± 0.16 | 3.11 ± 0.65 | 7.92 ± 0.69 | 0.76 ± 0.1 | |
94 | MK1 | 0.89 ± 0.04 | 1.71 ± 0.16 | 5.98 ± 0.10 | 0.79 ± 0.08 |
AMM pAD-GND1 | 1.79 ± 0.12 | 7.63 ± 0.48 | 18.54 ± 1.67 | 0 | |
AMM pAD-ZWF1 | 2.61 ± 0.16 | 7.02 ± 0.38 | 15.32 ± 4.28 | 0 | |
AMM pAD-TKL1 | 1.53 ± 0.39 | 6.66 ± 0.78 | 12.70 ± 2.18 | 0.31 ± 0.10 | |
AMM pAD-TAL1 | 2.30 ± 0.13 | 6.23 ± 0.36 | 11.16 ± 1.41 | 1.14 ± 0.52 |