Table 4 Side-metabolites of Y. lipolytica strains in shake-flask experiments in baffled flasks
From: Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica
Time (h) | Strain | Arabitol (g/L) | Mannitol (g/L) | Citric acid (g/L) | α-Ketoglutaric acid (g/L) |
|---|---|---|---|---|---|
24 | MK1 | 0.24 ± 0.03 | 0 | 2.38 ± 0.03 | 0.23 ± 0.01 |
AMM pAD-GND1 | 0.15 ± 0.04 | 0.29 ± 0.01 | 8.05 ± 0.05 | 0.25 ± 0.00 | |
AMM pAD-ZWF1 | 0.35 ± 0.01 | 0.37 ± 0.05 | 9.00 ± 1.30 | 0.33 ± 0.04 | |
AMM pAD-TKL1 | 0.26 ± 0.01 | 0.32 ± 0.03 | 7.61 ± 0.21 | 0.47 ± 0.02 | |
AMM pAD-TAL1 | 0 | 3.20 ± 0.05 | 7.10 ± 0.50 | 0 | |
48 | MK1 | 0.77 ± 0.00 | 1.19 ± 0.01 | 9.53 ± 0.36 | 0.44 ± 0.01 |
AMM pAD-GND1 | 0.70 ± 0.02 | 3.14 ± 0.02 | 14.26 ± 0.08 | 1.12 ± 0.08 | |
AMM pAD-ZWF1 | 1.05 ± 0.01 | 3.22 ± 0.13 | 15.37 ± 3.54 | 1.31 ± 0.24 | |
AMM pAD-TKL1 | 0.52 ± 0.00 | 2.43 ± 0.07 | 13.64 ± 0.54 | 2.10 ± 0.11 | |
AMM pAD-TAL1 | 0.93 ± 0.02 | 3.20 ± 0.05 | 17.03 ± 2.82 | 0.27 ± 0.19 | |
54 | MK1 | 0.76 ± 0.05 | 1.35 ± 0.27 | 9.99 ± 0.28 | 0.45 ± 0.01 |
AMM pAD-GND1 | 0.74 ± 0.02 | 3.70 ± 0.05 | 15.60 ± 0.24 | 1.10 ± 0.08 | |
AMM pAD-ZWF1 | 1.08 ± 0.02 | 3.79 ± 0.13 | 16.64 ± 4.04 | 1.29 ± 0.24 | |
AMM pAD-TKL1 | 0.54 ± 0.00 | 2.87 ± 0.05 | 15.61 ± 0.54 | 2.04 ± 0.13 | |
AMM pAD-TAL1 | 1.16 ± 0.07 | 4.73 ± 0.15 | 13.67 ± 1.77 | 0.00 ± 0.00 | |
72 | MK1 | 0.71 ± 0.07 | 1.90 ± 0.08 | 10.69 ± 0.27 | 0.37 ± 0.02 |
AMM pAD-GND1 | 0.75 ± 0.04 | 4.26 ± 0.10 | 15.95 ± 0.34 | 0 | |
AMM pAD-ZWF1 | 1.08 ± 0.10 | 4.27 ± 0.09 | 15.97 ± 3.83 | 0 | |
AMM pAD-TKL1 | 0.55 ± 0.01 | 3.40 ± 0.06 | 17.35 ± 1.60 | 0 | |
AMM pAD-TAL1 | 0.96 ± 0.25 | 4.72 ± 0.40 | 23.37 ± 0.92 | 0 |