Fig. 1

Characterization of Tab2 levels in wild type (CC125) and the tab2 mutant during the N deprivation time course. a Relative expression level of the TAB2 gene, normalized to the expression of Actin (see Additional file 1: Table S1) and then the relative abundance of each time point was normalized to time 0. b Abundance of the TAB2 protein, normalized to WT at time 0. Data are presented as mean ± SE (n = 3). *p < 0.05. c, d Volcano plots of overall proteomic changes in WT and the tab2 mutant, respectively, comparing fold change of proteins in nitrogen depleted (48 h) relative to nitrogen replete (time 0) conditions. Proteins with significant changes (p < 0.05) are shown by red ellipses. The stringent cutoff of p < 0.01 is shown by a dotted line