TY - JOUR AU - Wang, Chaogang AU - Chen, Xi AU - Li, Hui AU - Wang, Jiangxin AU - Hu, Zhangli PY - 2017 DA - 2017/04/13 TI - Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii JO - Biotechnology for Biofuels SP - 91 VL - 10 IS - 1 AB - Nutrient limitation, such as nitrogen depletion, is the most widely used method for improving microalgae fatty acid production; however, these harsh conditions also inhibit algal growth significantly and even kill cells at all. To avoid these problems, we used artificial microRNA (amiRNA) technology as a useful tool to manipulate metabolic pathways to increase fatty acid contents effectively in the green microalga Chlamydomonas reinhardtii. We down-regulated the expression of phosphoenolpyruvate carboxylase (PEPC), which catalyzes the formation of oxaloacetate from phosphoenolpyruvate and regulates carbon flux. SN - 1754-6834 UR - https://doi.org/10.1186/s13068-017-0779-z DO - 10.1186/s13068-017-0779-z ID - Wang2017 ER -