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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii

Fig. 5

Schematic diagram of the construct pH-amicroRNA-PEPC1/2. pH124 contained the ampicillin- and zeomycin-resistant genes and a strong heat-inducible promoter (Hsp70A-RBCS2). The DNA fragments of amicroRNA-PEPC1 (aRP1) and amicroRNA-PEPC2 (aRP2) were ligated to the restriction enzyme sites, NheI and PmaCI, within the Chlamydomonas transformation vector pH124, resulting in pH-amicroRNA-PEPC1 (pH-aRP1) and pH-amicroRNA-PEPC2 (pH-aRP2), respectively. The amiRNA precursors included amicroRNA-PEPC1/2, which were overexpressed under Hsp70A promoter

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