Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii

Wang, Chaogang; Chen, Xi; Li, Hui; Wang, Jiangxin; Hu, Zhangli

doi:10.1186/s13068-017-0779-z

Biotechnology for Biofuels and Bioproducts

Table 1 Fatty acids and TFA contents (% cell dry weight) in transgenic strains with amicroRNA-PEPCs as analyzed by GC–MS

From: Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii

Fatty acids	CC-849	aRP1.1	aRP1.2	aRP1.3	aRP1.4	aRP2.1	aRP2.2	aRP2.3
C12:0	0.046 ± 0.001	0.053 ± 0.001	0.059 ± 0.001	0.165 ± 0.029	0.046 ± 0.003	0.00	0.00	0.035 ± 0.031
C14:0	0.234 ± 0.004	0.29 ± 0.012	0.293 ± 0.022	0.369 ± 0.021	0.233 ± 0.032	0.254 ± 0.015	0.246 ± 0.022	0.314 ± 0.055
C16:0	15.46 ± 0.477	16.411 ± 0.685	18.063 ± 0.277^a	18.602 ± 1.161^a	15.054 ± 0.19	21.709 ± 1.005^a	21.268 ± 1.344^a	19.14 ± 1.667^a
C16:1	2.247 ± 0.033	3.162 ± 0.117^b	3.231 ± 0.465	3.849 ± 0.377^a	2.36 ± 0.212	2.571 ± 0.164	2.838 ± 0.117^a	2.727 ± 0.32
C18:0	2.12 ± 0.066	2.561 ± 0.085^a	2.22 ± 0.194	2.539 ± 0.18^a	2.08 ± 0.205	2.544 ± 0.165^a	2.115 ± 0.247	2.232 ± 0.286
C16:4	22.987 ± 0.097	25.535 ± 0.862^a	25.67 ± 1.452^a	25.852 ± 1.363^a	24.25 ± 1.418	25.057 ± 1.338	27.57 ± 0.696^b	28.82 ± 1.088^b
C18:1 t	0.818 ± 0.041	1.233 ± 0.108^b	1.769 ± 0.414	2.649 ± 0.633^a	0.91 ± 0.089	1.283 ± 0.109^b	1.235 ± 0.295	0.969 ± 0.165
C18:2 t	3.353 ± 0.343	4.253 ± 0.365^a	4.219 ± 0.081^a	4.242 ± 0.288^a	3.448 ± 0.211	4.189 ± 0.667	3.575 ± 0.596	5.034 ± 0.783^a
C18:3	5.806 ± 0.178	7.345 ± 0.49^b	5.696 ± 0.309	6.768 ± 0.358^a	5.688 ± 0.614	8.376 ± 0.586^b	6.059 ± 0.297	6.682 ± 1.092
C18:3n3	30.777 ± 0.465	31.353 ± 1.037	34.298 ± 2.817	32.517 ± 1.715	31.633 ± 2.849	32.311 ± 1.103	34.343 ± 1.283^a	36.74 ± 0.781
C20:1	1.504 ± 0.033	2.054 ± 0.046^b	1.555 ± 0.153	1.795 ± 0.095^b	1.599 ± 0.204	2.133 ± 0.032^b	1.946 ± 0.067^a	1.977 ± 0.246^b
C20:3	0.012 ± 0.003	0.089 ± 0.029^a	0.055 ± 0.074	0.086 ± 0.049	0.026 ± 0.026	0.00	0.00	0.065 ± 0.067
C20:4	0.031 ± 0.001	0.06 ± 0.002^b	0.042 ± 0.016	0.062 ± 0.004^b	0.042 ± 0.017	0.005 ± 0.002^b	0.007 ± 0.007^a	0.047 ± 0.029
C22:2	0.00	0.081 ± 0.057	0.00	0.052 ± 0.003^b	0.026 ± 0.037	0.011 ± 0.003^a	0.008 ± 0.002^a	0.08 ± 0.094
C24:0	0.085 ± 0.001	0.216 ± 0.014^b	0.146 ± 0.067	0.242 ± 0.015^b	0.141 ± 0.065	0.102 ± 0.004^b	0.105 ± 0.012^a	0.18 ± 0.067
C22:6	0.016 ± 0.004	0.093 ± 0.018^a	0.045 ± 0.061	0.089 ± 0.006^b	0.033 ± 0.045	0.014 ± 0.001	0.014 ± 0.002	0.074 ± 0.051
TFA	85.497 ± 1.581	94.788 ± 3.312^a	97.377 ± 3.06^a	99.878 ± 5.496^a	87.56 ± 3.208	100.561 ± 4.856^b	101.33 ± 4.842^b	105.193 ± 3.618^b

The table shows the average of all the observations from 4 repeated tests, in the form of mean ± standard error of representation, wild-type C. reinhardtii CC-849 represents a control, TFA represents the total fatty acid content. All data are expressed in mg/g (dry weight)
The data represent the means ± SD of three replicate experiments and were analyzed by Student’s t-test (n = 3)
^aP <0.05; ^bP <0.01. aRP1.1, aRP1.2, aRP1.3, aRP1.4 is transformed amicroRNA-PEPC1 into C. reinhardtii; aRP2.1, aRP2.2, aRP2.3 is transformed amicroRNA-PEPC2 into C. reinhardtii

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