Fig. 1

Schematic diagram of the construction of xlnR ^A871V, RE-6, RE-7, and RE-8 mutants. a Construction of the xlnR ^A871V cassette and identification of xlnR ^A871V. Replacement of xlnR by xlnR ^A871V expression cassette resulted in an increase from 6.5 kb in the wild-type strain to 8.4 kb in xlnR ^A871V strain with primer pair xmU-F + xmD-R. b Construction of ΔcreA-gpdA(p)::clrB cassette and identification of RE-6. The 2.8 and 1.6 kb fragments were detected in the RE-6, RE-7, and RE-8 strains with primer pairs creAU-F + gpdA-R and ptrA-YZF + creAD-R. c Construction of PDE_02864(p)::xlnR ^A871V cassette and identification of RE-7. The 5.1 kb fragment was detected in RE-7 stain with primer pair PDE_02864-F + xlnR-R. d Construction of PDE_02864(p)::xlnR ^A871V-cbh1-2(p)::cbh1-2-eg1(p)::eg1-pyrG cassette and identification of RE-8. The 4.5, 4.3, and 3.1 kb fragments were detected in RE-8 stain with x-YZF + cbh1-R, c-YZF + eg1-R, and e-YZF + six-pyrG-R primer pairs, respectively