Moderate alkali-thermophilic ethanologenesis by locally isolated Bacillus licheniformis from Pakistan employing sugarcane bagasse: a comparative aspect of aseptic and non-aseptic fermentations

Table 2 Ethanol yield of Bacillus licheniformis KU886221 using SCB hydrolysate

Serial no.	Pretreatment	Incubation (h)	Total sugars (g/L)^a	Ethanol (g/L)
1	2% H₂SO₄	0	41.952^bc ± 0.417	0^g
		24	35.913^ef ± 0.676	1.101^f ± 0.124
		72	27.811^gh ± 0.333	3.491^d ± 0.129
		120	25.464^hi ± 0.579	4.294^bc ± 0.315
2	3% H₃PO₃	0	38.405^de ± 0.569	0^g
		24	33.823^f ± 1.026	1.593^ef ± 0.143
		72	24.529ⁱ ± 0.580	4.204^bc ± 0.047
		120	16.769^k ± 0.299	4.5413^ab ± 0.068
3	1% HCl	0	44.919^ab ± 0.212	0^g
		24	41.214^cd ± 0.369	1.165^ef ± 0.179
		72	30.202^g ± 0.431	3.742^cd ± 0.080
		120	29.170^g ± 0.448	4.282^bc ± 0.053
4	1 N NaOH	0	46.306^a ± 0.465	0
		24	39.345^cd ± 0.126	1.483^ef ± 0.097
		72	28.981^g ± 0.306	3.955^bcd ± 0.163
		120	28.471 ^g ± 0.575	4.937^a ± 0.140
5	Control (untreated)	0	33.902^f ± 1.150	0^g
		24	29.061^g ± 0.658	0.420^g ± 0.079
		72	25.139^hi ± 0.282	1.681^ef ± 0.066
		120	21.527^j ± 0.545	1.962^e ± 0.090

^aGlucose + xylose. Values signify mean ± SE of triplicates. Values that do not share an alphabet are significantly different from each other. Multi-factor analysis of variance (P < 0.05)

ISSN: 2731-3654