Fig. 2

Molecular characterization of AGPAT1-overexpression microalgal transformants. a, PCR amplification of CAT gene from the genomic DNA of transformants. Lanes 1 and 2: transgenic lines, Lanes 3 and 4: negative control (wild-type genomic DNA and water, respectively); Lane M 5000 bp ladder marker. b Relative transcript abundance of AGPAT1 determined by qPCR. The results were normalized against β-actin gene. c AGPAT1 protein expression measured by Western blot analysis with anti-c-Myc antibody. β-actin was used as an internal control. d Enzymatic assay of AGPAT1. e Relative transcript level of GPAT by qPCR. The results were normalized against β-actin gene. f Relative transcript level of DGAT2 by qPCR. The results were normalized against β-actin gene. Significant difference between wild type and transgenic microalgae is indicated at *p < 0.05 or **p < 0.01 level. Each value represents mean ± SD (n = 3)