Fig. 1

Optimization of the refolding parameters for the recombinant IlMnP1 and IlMnP2. a pH. b Urea concentration. c GSSG concentration. d Hemin concentration. e Refolding time. All reactions were performed with 0.1 mg/mL of protein in 50 mM Tris–HCl buffer containing 5 mM Ca²⁺, 0.1 mM EDTA, and 0.1 mM DTT at 15 °C