Production of high-concentration n-caproic acid from lactate through fermentation using a newly isolated Ruminococcaceae bacterium CPB6

Table 3 Key possible reactions for caproic acid (CA) formation and stoichiometric fermentation balances using the crude cell extract of strain CPB6 grown on different substrates

	Acetate^a	Butyrate^a	Caproate^a
Caproate formation
Rec. (1) Caproyl-CoA + Acetate → Caproate + Acetyl-CoA	−1.15^b	nd^c	1.14
Rec. (2) Caproyl-CoA + Butyrate → Caproate + Butyryl-CoA	nd^c	−0.77^c	0.76
Butyrate formation
Rec. (3) Butyryl-CoA + Acetate → Butyrate + Acetyl-CoA	−0.45^c	0.44^c	nd^c

Supplement of substrates (acetate, butyrate, butyryl-CoA, and caproyl-CoA) were sufficient
Control measurements (without crude enzyme solution) were not shown for no butyrate or caproate was produced
^a The unit is mM
^b “−” Substrate consumption
^c Not detected

ISSN: 2731-3654