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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Fig. 3

Growth, glucose consumption and product formation in anaerobic bioreactor batch cultures of S. cerevisiae strains with different genetic modifications in glycerol and acetate metabolism. Cultures were grown on synthetic medium containing 180 g L−1 glucose and 3 g L−1 acetic acid (pH 5). a Strain IMX992 (GPD1 GPD2 sga1::eutE); b strain IMX884 (GPD1 gpd2::eutE); c strain IMX776 (gpd1::gpsA gpd2::eutE); d strain IMX901 (gpd1::gpsA gpd2::eutE ald6Δ). Closed circle glucose; closed square biomass; open square glycerol; open circle ethanol; open triangle acetate. ac display single representative cultures from a set of two independent duplicate cultures for each strain. In the culture of IMX901, acetic acid was added externally immediately after the exponential growth phase was finished

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