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Table 1 Saccharomyces cerevisiae strains used in this study

From: Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Strain name Relevant genotype Origin
IMX585 MAL2-8c SUC2 can1::cas9-natNT2 [48]
IMX581 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 [48]
IMZ160 ura3 leu2::LEU2 [pRS405] gpd1::loxP gpd2::hphMX4 pUDE43 [43]
IME324 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 p426-TEF (empty) This work
IMX884 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 gpd2::eutE pROS10 (GPD2-targeting) This work
IMX992 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 sga1::eutE pUDR119 This work
IMX776 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 gpd1::gpsA gpd2::eutE pUDR240 This work
IMX901 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 gpd1::gpsA gpd2::eutE ald6Δ pUDR240 This work
IMX888 MAL2-8c SUC2 can1::cas9-natNT2 gpd1Δ gpd2::eutE [29]
IMX900 MAL2-8c SUC2 can1::cas9-natNT2 gpd1Δ gpd2::eutE ald6Δ This work
IMX1039 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 gpd1::gpsA gpd2::eutE ald6Δ This work
IMX1120 MAL2-8c SUC2 can1::cas9-natNT2 gpd1Δ gpd2::eutE ald6Δ sga1::ALD6 This work
IMX1142 ura3-52 MAL2-8c SUC2 can1::cas9-natNT2 gpd1::gpsA gpd2::eutE ald6Δ sga1::ALD6 pUDR103 This work