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Table 3 Specific growth rates (μ) and stoichiometric relationships between glycerol production and biomass formation, acetate consumption and glucose consumption, and acetate consumption and biomass formation in anaerobic bioreactor batch cultures of S. cerevisiae strains with different genetic modifications in glycerol and acetate metabolism

From: Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Strain IME324 IMX992 IMX884 IMX776 IMX901 IMX888a
Relevant genotype GPD1
GPD2
GPD1
GPD2
sga1::eutE
GPD1
gpd2::eutE
gpd1::gpsA
gpd2::eutE
gpd1::gpsA
gpd2::eutE
ald6Δ
gpd1Δ
gpd2::eutE
μ (h−1) 0.31 ± 0.01 0.30 ± 0.00 0.31 ± 0.01 0.24 ± 0.01 0.24 ± 0.01 0.26 ± 0.01
Ratio glycerol produced/biomass [mmol (g biomass)−1] 9.19 ± 0.08 8.28 ± 0.14 1.92 ± 0.06 <0.1 <0.1 <0.1
Ratio acetate consumed/biomass [mmol (g biomass)−1] 2.43 ± 0.16 3.35 ± 0.08 5.77 ± 0.25 6.66 ± 0.01 6.41 ± 0.28 6.92 ± 0.12
Ratio acetate consumed/glucose (g g−1) 0.010 ± 0.000 0.015 ± 0.000 0.026 ± 0.001 0.031 ± 0.001 0.031 ± 0.000 0.032 ± 0.000
  1. Cultures were grown on synthetic medium containing 20 g L−1 glucose and 3 g L−1 acetic acid (pH 5). Specific growth rates and stoichiometries were calculated from the mid-exponential growth phase and represent averages ± mean deviations of data obtained from independent duplicate cultures. In all cultures, carbon recoveries were between 95 and 100%. Enzyme activities of acetylating-acetaldehyde dehydrogenase in cell extracts of eutE-expressing strains were similar (Additional file 2)
  2. aData on strain IMX888 were taken from [29]