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Table 4 Specific growth rates (μ), yields (Y) of biomass, ethanol and glycerol on glucose and stoichiometric relationships between glycerol production and biomass formation, acetate consumption and glucose consumption, and acetate consumption and biomass formation in anaerobic bioreactor batch cultures of S. cerevisiae strains with different genetic modifications in glycerol and acetate metabolism

From: Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Strain IMX992 IMX884 IMX776 IMX901
Relevant genotype GPD1 GPD2
sga1::eutE
GPD1
gpd2::eutE
gpd1::gpsA
gpd2::eutE
gpd1::gpsA
gpd2::eutE
ald6Δ
μ (h−1) 0.28 ± 0.02 0.27 ± 0.00 0.14 ± 0.00 0.12 ± 0.02
Y biomass/glucose (g g−1) 0.087 ± 0.001 0.085 ± 0.000 0.089 ± 0.000 0.077 ± 0.013
Y ethanol/glucose (g g−1) 0.43 ± 0.01 0.42 ± 0.02 0.47 ± 0.01 0.49 ± 0.00
Y glycerol/glucose (g g−1) 0.07 ± 0.00 0.05 ± 0.00 0.02 ± 0.00 <0.001
Glycerol produced/biomass [mmol (g biomass)−1] 8.76 ± 0.25 6.34 ± 0.26 3.29 ± 0.41 <0.1
Acetate consumed/biomass [mmol (g biomass)−1] 2.67 ± 0.96 2.98 ± 0.08 2.88 ± 0.17 5.71 ± 0.15
Acetate consumed/glucose (g g−1) 0.011 ± 0.001 0.015 ± 0.000 0.016 ± 0.000 0.027 ± 0.003
  1. Cultures were grown on synthetic medium containing 180 g L−1 glucose and 3 g L−1 acetic acid (pH 5). Specific growth rates and stoichiometries were calculated from the mid-exponential growth phase and represent averages ± mean deviations of data obtained from independent duplicate cultures