Fig. 8
Evolution of ROS in different yeast cell inocula (CEN.PK 113-7D) upon exposure to a mixture of lignocellulosic inhibitors. Representative histograms from the following inocula: a LP-cells, b cells pre-adapted in medium supplemented with the inhibitors at pH 5 and c ESP-cells. The vertical line displays cells with high and low ROS levels and was defined as the number (F585/40 = 52 × 104 channel number) separating the LP-cells (a, inoculum) into fractions of 1% lowest and 99% highest percentiles. d Mean F585/40 and e coefficient of variation (CV) of F585/40 for the three different inocula (LP-cells, grey; pre-adapted cells, blue; ESP-cells, green) measured over time. f Frequency of cells with low ROS levels over time. Error bars represent standard error of biological duplicates (n = 2). F585/40 is DHE fluorescence emission collected at 585/30 nm originating from excitation at 488 nm, respectively. Flow cytometry measurement was performed with a BD Accuri instrument and data analysis was performed with Flowjo. Histograms are from a representative experiment