Fig. 2
From: Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
SDS-PAGE analysis of cellulosomal (C) and extracellular proteins (E) of C. thermocellum strains. The parent strain ∆pyrF produced an intact CelS protein (black arrows). Compared to ∆pyrF, an additional ~135-kDa band was observed in both cellulosomal and extracellular proteins of ∆pyrF::CaBglA and ∆pyrF::CaBglAm (red arrows), suggesting the successful expression of the fusion protein Cel-CaBglA(m)-Doc, and its assembly in the cellulosome. Cellulosomal and extracellular proteins of ∆pyrF::CaBglA with the size of ~135 and ~75 kDa were further identified by mass spectroscopy (Additional file 3). Bands corresponding to known cellulosomal proteins are identified to the left of the Coomassie blue-stained gel. M protein standards