Fig. 5
From: Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
Saccharification and pH curves of C. thermocellum strain ∆pyrF::CaBglA with increased cell density. Cells from 200- to 300-mL culture were concentrated, resuspended, and reinoculated into 100 mL fresh GS-2 medium with 100 g/L Avicel as a sole carbon source to increase the cell density by 1.6 (red) or 2.4 fold (black). The concentration of produced reducing sugars (closed circles) and the pH values (open circles) were determined based on three independent replicates, and values are shown as average ± standard deviation