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Table 1 Enzymatic properties of selected BGLs

From: Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum

Protein Optimal temp (°C)/pHa Specific activity (U/mg) Thermal stability at 60/80 °C (%)b Glucose inhibition (mM)c
CaBglA 75–80/5.0–5.6 [23] 346.0 ± 3.7 99.3/91.4 314
CtBglA 65/5.5 208.2 ± 4.8 116.9/4.8 205
CglT 60–75/6.0–7.0 [25] 79.3 ± 5.9 104.0/10.5 450
Td2f2 75/5.5 [31] 9.2 ± 0.3 71.1/40.9 Noned
  1. All experiments were performed in triplicate to calculate the averages and standard errors with pNPG as a substrate, and the reaction conditions were at pH 5.5 and 55 °C
  2. a The optimal temperature and pH of CaBglA and CtBglA were also determined in this study as shown in Additional file 1
  3. b The thermal stability was shown as percentage of remaining BGL activity after incubating at 60 or 80 °C for 24 h
  4. c The glucose inhibition of BGLs was determined by adding glucose at different concentrations (0–600 mM) to the standard reaction mixture, and calculated as the glucose concentration required to inhibit 50% of initial BGL activity (Additional file 1)
  5. d Instead of inhibition, Td2f2 could be stimulated by 29.2–72.0% with the addition of 100–600 mM glucose, which was consistent with previous report [31]