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Table 2 Bacterial strains and plasmids used in this study

From: Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum

Strains/plasmids Relevant characteristic Sources
Strains
 E. coli
  DH5α f80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(r k , m + k ), phoA, supE44, l , thi-1, gyrA96, relA1 Transgen
  BL21(DE3) ompT gal dcm lon hsdS B (r B m B ) l (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) Transgen
 C. thermocellum
  DSM1313 LQ8, wild type stain DSMZ
  ΔpyrF Derived from DSM1313, with deleted pyrF gene This work
  ΔpyrF::CaBglA Derived from ΔpyrF, expressing CelS-bearing CaBglA This work
  ΔpyrF::CaBglAm Derived from ΔpyrF, expressing CelS-bearing CaBglAm This work
  ∆pyrF::pHK-CtBglA-Doc Derived from ΔpyrF, containing plasmid pHK-CtBglA-Doc This work
  ∆pyrF::pHK-CaBglA-Doc Derived from ΔpyrF, containing plasmid pHK-CaBglA-Doc This work
Plasmids
 pHK pNW33N derivative, E. coliC. thermocellum shuttle vector, CmR/TmR [51]
 pHK-∆pyrF pHK derivative, containing upstream and downstream regions of pyrF for pyrF deletion This work
 pHK-HR pHK derivative, containing pyrF cassette, tdk cassette, three regions of homology, and the target DNA sequence to knock in This work
 pHK-HR-CaBglA pHK-HR derivative for markerless knock-in of caBglA in the chromosome of DSM 1313 This work
 pHK-HR-CtBglA pHK-HR derivative for markerless knock-in of ctBglA in the chromosome of DSM 1313 This work
 pHK-HR-CaBglAm pHK-HR derivative for markerless knock-in of caBglAm in the chromosome of DSM 1313 This work
 pHK-CtBglA-Doc pHK-HR-CtBglA derivative for expression of CtBglA-Doc in DSM 1313, with the promoter and signal peptide region of CelS This work
 pHK-CaBglA-Doc pHK-HR-CaBglA derivative for expression of CaBglA-Doc in DSM 1313, with the promoter and signal peptide region of CelS This work
 pET28aNS Expression vector with N-terminal hexahistidine affinity tag, with modified multiple cloning sites [49]
 pET28aNS-CaBglA pET28aNS derivative for expression of CaBglA This work
 pET28aNS-CtBglA pET28aNS derivative for expression of CtBglA This work
 pET28aNS-CglT pET28aNS derivative for expression of CglT This work
 pET28aNS-Td2f2 pET28aNS derivative for expression of Td2f2 This work