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Table 1 Comparison of the in vivo PSII-independent contribution to H2 production under different conditions

From: H2 production pathways in nutrient-replete mixotrophic Chlamydomonas cultures under low light. Response to the commentary article “On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions,” by Alberto Scoma and Szilvia Z. Tóth

Reference

In vivo PSII-independent contributiona (%)

Media

Strain

Cell densityb

Purged cultures

PARc

Notes

Healey [11]

100

NR

C. moewusii

ICC 97

  

300 (lux)

Dark–light cycle adaptation

 

100

NR

C. dysosmos

ICC 242

  

400 (lux)

Dark–light cycle adaptation

Gibbs et al. [10]

18

NR

F60

 

Yes

100 W/m2

Dark adaptation (2 h)

DCMU and acetate added simultaneously

Acetate uptake is inhibited by 91% in the presence of DCMU

Scoma et al. [2]

86d

NR

L159I-N230Y

(D1 mutant)

80

Yes

100

DCMU added after 150 h

2X acetate in TAP formulation

In cultures without DCMU, O2 accumulated in the headspaces

Jurado-Oller et al. [3]

21

NR

704

10

No

12

DCMU added at 0 h

Atmospheric O2 level when DCMU is added

O2 levels remained very low after 24 h

36

NR

704

10

Yes

12

DCMU added at 0 h

81

NR

704

10

No

12

DCMU added at 0 h

Atmospheric O2 level when DCMU is added

Cultures aerated every 24 h

Hemschemeier et al. [8]

0

-S

cc124

20

No

100

DCMU immediately after S depletion

40

-S

cc124

20

No

100

DCMU added 17 h after S depletion

80–65

-S

cc124

20

No

100

DCMU added during H2 production phase

H2 measured by MIMS

40

-S

cc124

27

No

100

DCMU added during H2 production phase

H2 measured by MIMS

100

-S

cc124

17

No

100

DCMU added during H2 production phase

H2 measured by MIMS

Fouchard et al. [7]

≈0

-S

cc124

5 × 106 cells/ml

Yes

110

DCMU immediately after S depletion

O2 levels near 0% when DCMU is added

20

-S

cc124

5 × 106 cells/ml

Yes

110

DCMU was added 24 h after S depletion

O2 levels near 10% when DCMU is added

Laurinavichene et al. [13]

51

-S

cc124

20–28

Yes

30

DCMU added after 46 h of S depletion

H2 production is maximal as this time

32

-S

cc124

20–28

Yes

30

DCMU added after 70 h of S depletion

28

-S

cc124

20–28

Yes

30

DCMU added after 94 h of S depletion

Chochois et al. [12]

10

-S

330

4 × 106 cells/ml

Yes

200

DCMU added 24 h after S depletion

Antalet al. [15]

30

-S

cc124

4 × 106 cells/ml

Yes

25

 

Philips et al. [5]

100

-N

cc124

5 × 106 cells/ml

No

60

DCMU added 72 h after N depletion

Volgusheva et al. [6]

26

-Mg

cc124

7

No

80

DCMU added 8 days after Mg depletion

O2 levels near 0 when DCMU is added

  1. NR Nutrient Replete
  2. aMeasured as the % of H2 production in the presence of DCMU (relative to control cultures)
  3. bIn mg chl. L−1 unless otherwise indicated
  4. cPhotosynthetic active radiation (PAR) in µmol photons m−2 s−1, unless otherwise indicated
  5. dData according to Table 2 in original publication