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Table 1 Comparison of the in vivo PSII-independent contribution to H2 production under different conditions

From: H2 production pathways in nutrient-replete mixotrophic Chlamydomonas cultures under low light. Response to the commentary article “On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions,” by Alberto Scoma and Szilvia Z. Tóth

Reference In vivo PSII-independent contributiona (%) Media Strain Cell densityb Purged cultures PARc Notes
Healey [11] 100 NR C. moewusii
ICC 97
   300 (lux) Dark–light cycle adaptation
  100 NR C. dysosmos
ICC 242
   400 (lux) Dark–light cycle adaptation
Gibbs et al. [10] 18 NR F60   Yes 100 W/m2 Dark adaptation (2 h)
DCMU and acetate added simultaneously
Acetate uptake is inhibited by 91% in the presence of DCMU
Scoma et al. [2] 86d NR L159I-N230Y
(D1 mutant)
80 Yes 100 DCMU added after 150 h
2X acetate in TAP formulation
In cultures without DCMU, O2 accumulated in the headspaces
Jurado-Oller et al. [3] 21 NR 704 10 No 12 DCMU added at 0 h
Atmospheric O2 level when DCMU is added
O2 levels remained very low after 24 h
36 NR 704 10 Yes 12 DCMU added at 0 h
81 NR 704 10 No 12 DCMU added at 0 h
Atmospheric O2 level when DCMU is added
Cultures aerated every 24 h
Hemschemeier et al. [8] 0 -S cc124 20 No 100 DCMU immediately after S depletion
40 -S cc124 20 No 100 DCMU added 17 h after S depletion
80–65 -S cc124 20 No 100 DCMU added during H2 production phase
H2 measured by MIMS
40 -S cc124 27 No 100 DCMU added during H2 production phase
H2 measured by MIMS
100 -S cc124 17 No 100 DCMU added during H2 production phase
H2 measured by MIMS
Fouchard et al. [7] ≈0 -S cc124 5 × 106 cells/ml Yes 110 DCMU immediately after S depletion
O2 levels near 0% when DCMU is added
20 -S cc124 5 × 106 cells/ml Yes 110 DCMU was added 24 h after S depletion
O2 levels near 10% when DCMU is added
Laurinavichene et al. [13] 51 -S cc124 20–28 Yes 30 DCMU added after 46 h of S depletion
H2 production is maximal as this time
32 -S cc124 20–28 Yes 30 DCMU added after 70 h of S depletion
28 -S cc124 20–28 Yes 30 DCMU added after 94 h of S depletion
Chochois et al. [12] 10 -S 330 4 × 106 cells/ml Yes 200 DCMU added 24 h after S depletion
Antalet al. [15] 30 -S cc124 4 × 106 cells/ml Yes 25  
Philips et al. [5] 100 -N cc124 5 × 106 cells/ml No 60 DCMU added 72 h after N depletion
Volgusheva et al. [6] 26 -Mg cc124 7 No 80 DCMU added 8 days after Mg depletion
O2 levels near 0 when DCMU is added
  1. NR Nutrient Replete
  2. aMeasured as the % of H2 production in the presence of DCMU (relative to control cultures)
  3. bIn mg chl. L−1 unless otherwise indicated
  4. cPhotosynthetic active radiation (PAR) in µmol photons m−2 s−1, unless otherwise indicated
  5. dData according to Table 2 in original publication