Biotechnology for Biofuels and Bioproducts

Table 3 Glycosidic linkage composition of Fs-DACS fractions after separation by C18 SPE and isolation in 67% (v/v) ethanol

From: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Linkage	mol%
	F0s	F0i	F30s	F30i	SEC pools from F0s				SEC pools from F30s
	F0s	F0i	F30s	F30i	A1–A2	A3–A7	A8–A13	A14–A15	B1–B6	B7–B14	B15–B17
2,4-rha	0	1	0	0	1	0	0	0	0	0	0
Total rha	0	1	0	0	1	0	0	0	0	0	0
t-ara	2	13	11	14	8	2	1	1	18	7	2
2-ara	1	6	4	8	1	0	2	0	6	4	1
3-ara	1	5	2	5	1	1	1	0	4	2	0
5-ara	0	7	3	4	6	0	0	1	5	1	0
2,5-ara	1	2	2	3	3	2	0	0	3	2	0
3,5-ara	0	1	0	0	0	0	0	0	0	0	0
2,3,5-ara	0	1	0	0	0	0	0	0	0	0	0
Total ara	4	34	23	33	19	6	4	3	36	16	4
t-xyl	24	10	21	11	6	10	19	46	12	19	33
2-xyl	10	6	6	5	4	19	7	8	5	8	4
3-xyl	1	1	1	1	0	0	2	2	1	2	2
4-xyl	21	7	16	11	13	43	21	4	14	18	10
2,3-xyl	0	1	1	1	1	1	0	0	2	1	1
3,4-xyl	1	11	6	16	3	3	0	0	11	5	0
2,3,4-xyl	0	7	3	8	1	1	0	0	6	2	0
Total xyl	58	41	55	53	29	77	50	59	50	54	50
t-man	3	1	0	0	1	2	4	3	0	0	0
4-man	1	0	0	0	0	0	1	1	0	0	0
Total man	4	1	0	0	1	2	5	4	0	0	0
t-gal	2	9	3	7	3	1	2	1	4	3	1
3-gal	0	2	0	1	0	0	0	0	1	1	0
4-gal	0	2	0	1	1	0	0	0	1	0	0
6-gal	0	1	0	1	1	0	0	0	0	0	0
Total gal	2	14	4	9	5	1	3	1	6	4	2
t-glc	9	2	3	1	5	3	9	12	1	6	2
2-glc	6	1	1	0	0	1	6	9	0	1	0
3-glc	1	0	0	0	0	0	1	1	0	0	0
4-glc	8	1	4	0	1	1	9	6	1	6	10
6-glc	6	2	5	1	34	7	12	5	4	8	20
3,6-glc	0	0	0	0	1	0	0	0	0	0	0
4,6-glc	0	0	3	0	0	0	1	0	0	3	11
Total glc	31	6	16	3	42	12	37	33	7	25	43
Total	99	96	99	98	97	99	99	99	98	99	100

Only neutral glycosyl residues were analysed. A glycosyl residue at the reducing end resulted in the same partially methylated alditol acetates as an internal glycosyl residue
t terminal, rha rhamnose, ara arabinose, xyl xylose, man mannose, gal galactose, glc glucose

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