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Table 5 List of oligosaccharides identified in F0s based on UHPLC-MS elution and fragmentation patterns

From: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Peak no Retention time (min) m/z [M + 2AA] MS2 fragmentsa Annotationb
1 7.67 624 282, 462, 444, 238, 300, 204, 221, 220, 178, 202 H3
2 8.32 594 282, 238, 462, 204, 300, 444, 414, 432, 178, 190, 202 H2P(H)
3 9.89 594 300, 282, 462, 238, 204, 414, 432, 256, 178, 202, 176, 444, 191, 172, 498 H2P(H)
4 10.86 710 534, 648, 666, 576 P3U
4 10.86 786 383, 282, 300, 462, 444, 624, 238, 690, 384, 606, 221, 324, 750 H4
5 11.11 624 462, 282, 204, 444, 300, 463, 238, 221, 528, 342, 192 H3
6 12.08 710 534, 648, 666, 576 P3U/U2XXc
6 12.13  594 204, 300, 282, 462, 414, 252, 432, 221, 444, 256, 176, 270 H2P(H)
6 12.28 709 534, 576, 516, 402, 384 P3A
7 12.76 948 786, 624, 768, 828, 383, 462, 606, 282 H5
8 13.16 564 432, 252, 270, 226, 176, 204, 414, 162, 160, 171, 402, 172, 208 HP2(P)
8 13.16 710 534, 648, 666, 578 P3U
9 13.44 754 564, 678, 722, 606, 710 HP2Ume(H)
10 14.26 786 624, 462, 606, 282, 300, 383, 444 H4
11 14.55 723 691, 576, 534, 692, 516, 402, 384 P3Ame
12 15.75 724 534, 648, 692, 576, 680 P3Ume/U4m2XXc
13 16.61 691 576, 534, 516, 402, 604 P3Au
14 17.04 666 534, 516, 384, 226, 252, 402, 270 P4
14 17.04 692 534, 648, 576, 630, 516 P3Uu
14 17.04 724 534, 648, 692, 576, 680, 516 P3Ume
15 17.88 578 402, 516, 534, 270 P2U/U2Xc
16 18.71 594 462, 300, 282, 238, 256, 204, 444, 178, 202, 176 H2P(H)
16 18.71 724 534, 648, 692, 576, 680 P3Ume
17 20.83 591 559, 402, 444, 252, 384, 472, 270, 226 P2A
17 20.67 696 534, 402, 270, 226, 516, 252, 384 HP3(P)
18 22.13 592 402, 516, 560, 548, 444, 252, 384 P2Ume/U4m2Xc
19 23.01 594 282, 300, 238, 462, 191, 204, 178, 256, 202, 176, 172, 444, 498, 220 H2P(H)
20 23.58 724 534, 692, 648, 576 P3Ume
21 23.94 534 384, 402, 162, 336, 204, 208, 226, 176, 252, 160 P3
22 24.41 726 414, 576, 432, 594, 396, 238, 264, 558, 282 H2P2(H)
23 28.24 828 666, 534, 648, 402, 270 HP4(P)
24 28.87 534 402, 226, 466, 252, 270, 160, 176, 384 P3
25 29.16 666 534, 226, 402, 270, 516, 252, 384 P4
26 29.49 534 402, 270, 226, 160, 252, 384, 176, 204, 162 P3
  1. The peak numbers are according to Fig. 3b
  2. aThe fragments are listed in the order of decreasing relative abundance. The first one is the base peak. Only fragments above 5% relative abundance are listed. Fragments containing the 2-AA label and the reducing end are printed in bold
  3. b H hexose, P pentose, U uronic acid, U me 4-O-methyl uronic acid, U u hexenuronic acid, A uronamide, A me 4-O-methyl uronamide, A u hexenuronamide. If pentosyl and hexosyl residues were present in an oligosaccharide, the reducing end is indicated in brackets
  4. cAnnotation based on heteroxylan nomenclature [15]