Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Jonathan, M. C.; DeMartini, J.; Van Stigt Thans, S.; Hommes, R.; Kabel, M. A.

doi:10.1186/s13068-017-0803-3

Biotechnology for Biofuels and Bioproducts

Table 5 List of oligosaccharides identified in F0s based on UHPLC-MS elution and fragmentation patterns

From: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Peak no	Retention time (min)	m/z [M + 2AA]⁻	MS² fragments^a	Annotation^b
1	7.67	624	282, 462, 444, 238, 300, 204, 221, 220, 178, 202	H₃
2	8.32	594	282, 238, 462, 204, 300, 444, 414, 432, 178, 190, 202	H₂P(H)
3	9.89	594	300, 282, 462, 238, 204, 414, 432, 256, 178, 202, 176, 444, 191, 172, 498	H₂P(H)
4	10.86	710	534, 648, 666, 576	P₃U
4	10.86	786	383, 282, 300, 462, 444, 624, 238, 690, 384, 606, 221, 324, 750	H₄
5	11.11	624	462, 282, 204, 444, 300, 463, 238, 221, 528, 342, 192	H₃
6	12.08	710	534, 648, 666, 576	P₃U/U²XX^c
6	12.13	594	204, 300, 282, 462, 414, 252, 432, 221, 444, 256, 176, 270	H₂P(H)
6	12.28	709	534, 576, 516, 402, 384	P₃A
7	12.76	948	786, 624, 768, 828, 383, 462, 606, 282	H₅
8	13.16	564	432, 252, 270, 226, 176, 204, 414, 162, 160, 171, 402, 172, 208	HP₂(P)
8	13.16	710	534, 648, 666, 578	P₃U
9	13.44	754	564, 678, 722, 606, 710	HP₂U_me(H)
10	14.26	786	624, 462, 606, 282, 300, 383, 444	H₄
11	14.55	723	691, 576, 534, 692, 516, 402, 384	P₃A_me
12	15.75	724	534, 648, 692, 576, 680	P₃U_me/U^4m2XX^c
13	16.61	691	576, 534, 516, 402, 604	P₃A_u
14	17.04	666	534, 516, 384, 226, 252, 402, 270	P₄
14	17.04	692	534, 648, 576, 630, 516	P₃U_u
14	17.04	724	534, 648, 692, 576, 680, 516	P₃U_me
15	17.88	578	402, 516, 534, 270	P₂U/U²X^c
16	18.71	594	462, 300, 282, 238, 256, 204, 444, 178, 202, 176	H₂P(H)
16	18.71	724	534, 648, 692, 576, 680	P₃U_me
17	20.83	591	559, 402, 444, 252, 384, 472, 270, 226	P₂A
17	20.67	696	534, 402, 270, 226, 516, 252, 384	HP₃(P)
18	22.13	592	402, 516, 560, 548, 444, 252, 384	P₂U_me/U^4m2X^c
19	23.01	594	282, 300, 238, 462, 191, 204, 178, 256, 202, 176, 172, 444, 498, 220	H₂P(H)
20	23.58	724	534, 692, 648, 576	P₃U_me
21	23.94	534	384, 402, 162, 336, 204, 208, 226, 176, 252, 160	P₃
22	24.41	726	414, 576, 432, 594, 396, 238, 264, 558, 282	H₂P₂(H)
23	28.24	828	666, 534, 648, 402, 270	HP₄(P)
24	28.87	534	402, 226, 466, 252, 270, 160, 176, 384	P₃
25	29.16	666	534, 226, 402, 270, 516, 252, 384	P₄
26	29.49	534	402, 270, 226, 160, 252, 384, 176, 204, 162	P₃

The peak numbers are according to Fig. 3b
^aThe fragments are listed in the order of decreasing relative abundance. The first one is the base peak. Only fragments above 5% relative abundance are listed. Fragments containing the 2-AA label and the reducing end are printed in bold
^b H hexose, P pentose, U uronic acid, U _me 4-O-methyl uronic acid, U _u hexenuronic acid, A uronamide, A _me 4-O-methyl uronamide, A _u hexenuronamide. If pentosyl and hexosyl residues were present in an oligosaccharide, the reducing end is indicated in brackets
^cAnnotation based on heteroxylan nomenclature [15]

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