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Table 5 List of oligosaccharides identified in F0s based on UHPLC-MS elution and fragmentation patterns

From: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Peak no

Retention time (min)

m/z [M + 2AA]

MS2 fragmentsa

Annotationb

1

7.67

624

282, 462, 444, 238, 300, 204, 221, 220, 178, 202

H3

2

8.32

594

282, 238, 462, 204, 300, 444, 414, 432, 178, 190, 202

H2P(H)

3

9.89

594

300, 282, 462, 238, 204, 414, 432, 256, 178, 202, 176, 444, 191, 172, 498

H2P(H)

4

10.86

710

534, 648, 666, 576

P3U

4

10.86

786

383, 282, 300, 462, 444, 624, 238, 690, 384, 606, 221, 324, 750

H4

5

11.11

624

462, 282, 204, 444, 300, 463, 238, 221, 528, 342, 192

H3

6

12.08

710

534, 648, 666, 576

P3U/U2XXc

6

12.13 

594

204, 300, 282, 462, 414, 252, 432, 221, 444, 256, 176, 270

H2P(H)

6

12.28

709

534, 576, 516, 402, 384

P3A

7

12.76

948

786, 624, 768, 828, 383, 462, 606, 282

H5

8

13.16

564

432, 252, 270, 226, 176, 204, 414, 162, 160, 171, 402, 172, 208

HP2(P)

8

13.16

710

534, 648, 666, 578

P3U

9

13.44

754

564, 678, 722, 606, 710

HP2Ume(H)

10

14.26

786

624, 462, 606, 282, 300, 383, 444

H4

11

14.55

723

691, 576, 534, 692, 516, 402, 384

P3Ame

12

15.75

724

534, 648, 692, 576, 680

P3Ume/U4m2XXc

13

16.61

691

576, 534, 516, 402, 604

P3Au

14

17.04

666

534, 516, 384, 226, 252, 402, 270

P4

14

17.04

692

534, 648, 576, 630, 516

P3Uu

14

17.04

724

534, 648, 692, 576, 680, 516

P3Ume

15

17.88

578

402, 516, 534, 270

P2U/U2Xc

16

18.71

594

462, 300, 282, 238, 256, 204, 444, 178, 202, 176

H2P(H)

16

18.71

724

534, 648, 692, 576, 680

P3Ume

17

20.83

591

559, 402, 444, 252, 384, 472, 270, 226

P2A

17

20.67

696

534, 402, 270, 226, 516, 252, 384

HP3(P)

18

22.13

592

402, 516, 560, 548, 444, 252, 384

P2Ume/U4m2Xc

19

23.01

594

282, 300, 238, 462, 191, 204, 178, 256, 202, 176, 172, 444, 498, 220

H2P(H)

20

23.58

724

534, 692, 648, 576

P3Ume

21

23.94

534

384, 402, 162, 336, 204, 208, 226, 176, 252, 160

P3

22

24.41

726

414, 576, 432, 594, 396, 238, 264, 558, 282

H2P2(H)

23

28.24

828

666, 534, 648, 402, 270

HP4(P)

24

28.87

534

402, 226, 466, 252, 270, 160, 176, 384

P3

25

29.16

666

534, 226, 402, 270, 516, 252, 384

P4

26

29.49

534

402, 270, 226, 160, 252, 384, 176, 204, 162

P3

  1. The peak numbers are according to Fig. 3b
  2. aThe fragments are listed in the order of decreasing relative abundance. The first one is the base peak. Only fragments above 5% relative abundance are listed. Fragments containing the 2-AA label and the reducing end are printed in bold
  3. b H hexose, P pentose, U uronic acid, U me 4-O-methyl uronic acid, U u hexenuronic acid, A uronamide, A me 4-O-methyl uronamide, A u hexenuronamide. If pentosyl and hexosyl residues were present in an oligosaccharide, the reducing end is indicated in brackets
  4. cAnnotation based on heteroxylan nomenclature [15]