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Table 6 List of oligosaccharides identified in F30s based on UHPLC-MS elution and fragmentation patterns

From: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Peak no. Retention time (min) m/z [M + 2AA] MS fragmentsa Annotationb
1 6.59 786 624, 606, 282, 300, 238 H4
1 6.59 948 786, 768, 300, 462, 282, 444, 624, 606 H5
2 8.64 1356 1224, 1194, 1062, 1206, 930, 1092, 828, 1176, 1044 HP8 (P)
3 9.59 930 798, 780, 666, 402 P6
4 9.89 1050 918, 888, 900, 594, 576, 870, 756, 738, 353, 300, 444, 1014, 930, 954 H4P2(H)
4 10.01 1062 930, 912, 798, 780, 666 P7
5 10.54 888 756, 594, 738, 576, 300, 282, 842 H3P2(H)
5 10.54 1050 756, 738, 918, 888, 870, 353, 300, 900 H4P2(H)/LXGc
6 11.1 756 594, 353, 282, 576, 300, 624 H3P(H)/LGc
7 11.68 888 726, 738, 708, 414, 353, 756, 576, 432, 594, 660 H3P2(H)/LXc
8 12.08 1050 888,756, 738, 870, 918, 353 H4P2(H)/XLGc
9 12.8 888 756, 594, 738, 576, 300, 282 H3P2(H)/XXGc
9 12.8 1020 888, 870, 594, 726, 432, 576, 300 H3P3(H)
10 13.48 1050 918, 900, 756, 738, 462, 300, 444 H4P2(H)
11 13.66 930 798, 780, 648, 666, 534, 516 P6
12 14.37 960 798, 666, 828, 534, 780, 648 HP5(P)
13 14.48 930 798, 666, 780 P6
14 14.58 1050 918, 462, 756, 738, 900, 444 H4P2(H)
15 15.39 1092 798, 960, 666, 942, 534, 780, 648 HP6(P)
16 15.93 1050 918, 900, 738, 462, 756, 444, 300 H4P2(H)
16 15.93 1182 1020, 888, 870, 1050, 1002, 432, 726 H4P3(H)/LXXc
17 16.63 798 666, 648 P5
17 16.63 902 756, 499, 594, 738, 367, 300 H3PD(H)/FGc
17 16.76 1020 888, 726, 432, 708, 870, 414, 560, 474 H3P3(H)
18 17.63 726 414, 576, 432, 594, 282, 238, 264, 558, 444 H2P2(H)
19 18.36 1020 888, 726, 708, 870, 432, 414 H3P3(H)
20 18.75 1196 1050, 888, 902, 884, 1032, 499, 756, 738, 1064, 870 H4P2D(H)/XFGc
21 19.35 798 666, 648 P5
22 21.93 1020 888, 726, 432, 708, 870, 952, 414, 560, 986 H3P3(H)
23 22.32 990 666, 828, 534, 810, 648, 402 H2P4(H)
24 24.6 828 666, 534, 648, 402, 270, 516 HP4(P)
25 25.84 960 666, 828, 534, 810, 648, 402, 485 HP5(P)
26 26.38 666 534, 402, 226, 516, 270, 252 P4
27 27.78 812 666, 534, 648 P4D
  1. aThe fragments are listed in the order of decreasing relative abundance. The first one is the base peak. Only fragments above 5% relative abundance are listed. Fragments containing the 2-AA label and the reducing end are printed in bold
  2. b H hexose, P pentose, D deoxyhexose. If pentosyl and hexosyl residues were present in an oligosaccharide, the reducing end is indicated in brackets
  3. cAnnotation according to xyloglucan nomenclature [20]