Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Jonathan, M. C.; DeMartini, J.; Van Stigt Thans, S.; Hommes, R.; Kabel, M. A.

doi:10.1186/s13068-017-0803-3

Biotechnology for Biofuels and Bioproducts

Table 6 List of oligosaccharides identified in F30s based on UHPLC-MS elution and fragmentation patterns

From: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

Peak no.	Retention time (min)	m/z [M + 2AA]⁻	MS fragments^a	Annotation^b
1	6.59	786	624, 606, 282, 300, 238	H₄
1	6.59	948	786, 768, 300, 462, 282, 444, 624, 606	H₅
2	8.64	1356	1224, 1194, 1062, 1206, 930, 1092, 828, 1176, 1044	HP₈ (P)
3	9.59	930	798, 780, 666, 402	P₆
4	9.89	1050	918, 888, 900, 594, 576, 870, 756, 738, 353, 300, 444, 1014, 930, 954	H₄P₂(H)
4	10.01	1062	930, 912, 798, 780, 666	P₇
5	10.54	888	756, 594, 738, 576, 300, 282, 842	H₃P₂(H)
5	10.54	1050	756, 738, 918, 888, 870, 353, 300, 900	H₄P₂(H)/LXG^c
6	11.1	756	594, 353, 282, 576, 300, 624	H₃P(H)/LG^c
7	11.68	888	726, 738, 708, 414, 353, 756, 576, 432, 594, 660	H₃P₂(H)/LX^c
8	12.08	1050	888,756, 738, 870, 918, 353	H₄P₂(H)/XLG^c
9	12.8	888	756, 594, 738, 576, 300, 282	H₃P₂(H)/XXG^c
9	12.8	1020	888, 870, 594, 726, 432, 576, 300	H₃P₃(H)
10	13.48	1050	918, 900, 756, 738, 462, 300, 444	H₄P₂(H)
11	13.66	930	798, 780, 648, 666, 534, 516	P₆
12	14.37	960	798, 666, 828, 534, 780, 648	HP₅(P)
13	14.48	930	798, 666, 780	P₆
14	14.58	1050	918, 462, 756, 738, 900, 444	H₄P₂(H)
15	15.39	1092	798, 960, 666, 942, 534, 780, 648	HP₆(P)
16	15.93	1050	918, 900, 738, 462, 756, 444, 300	H₄P₂(H)
16	15.93	1182	1020, 888, 870, 1050, 1002, 432, 726	H₄P₃(H)/LXX^c
17	16.63	798	666, 648	P₅
17	16.63	902	756, 499, 594, 738, 367, 300	H₃PD(H)/FG^c
17	16.76	1020	888, 726, 432, 708, 870, 414, 560, 474	H₃P₃(H)
18	17.63	726	414, 576, 432, 594, 282, 238, 264, 558, 444	H₂P₂(H)
19	18.36	1020	888, 726, 708, 870, 432, 414	H₃P₃(H)
20	18.75	1196	1050, 888, 902, 884, 1032, 499, 756, 738, 1064, 870	H₄P₂D(H)/XFG^c
21	19.35	798	666, 648	P₅
22	21.93	1020	888, 726, 432, 708, 870, 952, 414, 560, 986	H₃P₃(H)
23	22.32	990	666, 828, 534, 810, 648, 402	H₂P₄(H)
24	24.6	828	666, 534, 648, 402, 270, 516	HP₄(P)
25	25.84	960	666, 828, 534, 810, 648, 402, 485	HP₅(P)
26	26.38	666	534, 402, 226, 516, 270, 252	P₄
27	27.78	812	666, 534, 648	P₄D

^aThe fragments are listed in the order of decreasing relative abundance. The first one is the base peak. Only fragments above 5% relative abundance are listed. Fragments containing the 2-AA label and the reducing end are printed in bold
^b H hexose, P pentose, D deoxyhexose. If pentosyl and hexosyl residues were present in an oligosaccharide, the reducing end is indicated in brackets
^cAnnotation according to xyloglucan nomenclature [20]

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