Table 1 List of selected genes, PCR primers, and predicted size
Fosmid name | Predicted enzyme (Gene encoding) | Gene no. | Primers | Restriction enzymes | Size (bp) | Approx. size (kDa) | Closet hit (identity/coverage) |
|---|---|---|---|---|---|---|---|
NT2-2 | β-Galactosidase | 1 | FP-TACgaattcACGGCATCAGCGCAATAGTC RP-AGggatccCGCTTCGCAACTGAGGTTAC | EcoRI BamHI | 3146 | 116 | Enterobacter hormaechei (74/81%) |
T5-5 | β-Xylosidase | 2 | FP-GCGaagcttGTGTAGTCTTTGCCCATCTC RP-CAGggatccCGCTTCGCAACTGAGGTTAC | HindIII BamHI | 2410 | 85 | Enterobacter mori (83/90%) |
T4-1 | β-Xylosidase | 3 | FP-GCGgaattcCACCAGATTCAGCCCTACAG RP-CAGaagcttTGTAGTCTTTGCCCATCTCC | EcoRI HindIII | 2439 | 86 | Enterobacter mori (83/90%) |
10BT | Transcription regulator | 4 | FP-GCGggatccGCACAACAAGATGGCTTTAC RP-CAGctcgagTAGTCGCATTTTGACGGAAC | BamHI XhoI | 627 | 22 | Klebsiella oxytoca (76/87%) |
Inner membrane protein | 5 | FP-GCGaagcttGTGCAAAGTATCGCTTTAAC RP-CAGggatccAGCAGCATGAATAAGGACAC | HindIII BamHI | 1262 | 45 | Enterobacter cloacae (79/88%) | |
NT18-17 | Aquaporin | 6 | FP-GCGaagcttGGGATCGGCTAGATCTTGAG RP-CAGggatccCAAGCGTCATGAATAAACTG | HindIII BamHI | 673 | 22 | Hyphomonas neptunium (65/80%) |
Beta-hexosaminidase | 7 | FP-GCGggatccTTTGCGGAGCCTACTCCTTC RP-CAGaagcttTTACAATGGCATCTGATTGG | BamHI HindIII | 1944 | 70 | Rhizobium leguminosarum 3841 (50/64%) | |
Hypothetical protein | 8 | FP-GCGggatccCCGGATACTCCACACAAAGG RP-CAGctcgagTTATCGCAAGTCGATCAAGG | BamHI XhoI | 1134 | 37 | Hyphomicrobium denitrificans (56/71%) |