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Table 1 Outcome of the ALE

From: Efficient itaconic acid production from glycerol with Ustilago vetiveriae TZ1

 

Growth (h−1)

Glycerol rate (g L−1 h−1)

Itaconate titer (g L−1)

Itaconate rate (g L−1 h−1)

Malate titer (g L−1)

Malate rate (g L−1 h−1)

U. vetiveriae RK 075

0.05 ± 0.00 (0.07 ± 0.00)

0.30 ± 0.01 (0.31 ± 0.03)

4.4 ± 0.8 (10.4 ± 2.1)

0.01 ± 0.00 (0.03 ± 0.01)

11.5 ± 0.5 (26.6 ± 4.7)

0.03 ± 0.00 (0.07 ± 0.01)

U. xerochloae UMa702

0.05 ± 0.00 (0.05 ± 0.00)

0.35 ± 0.0 (0.63 ± 0.00)

20.1 ± 4.6 (0.0 ± 0.0)

0.05 ± 0.01 (0.00 ± 0.00)

10.5 ± 0.7 (81.2 ± 8.4)

0.03 ± 0.00 (0.21 ± 0.02)

Macalpinomyces mackinlayi BRIP 52549 a

0.05 ± 0.00 (0.05 ± 0.00)

0.38 ± 0.00 (0.64 ± 0.01)

0.0 ± 0.0 (0.0 ± 0.0)

0.00 ± 0.00 (0.00 ± 0.00)

63.1 ± 0.3 (35.5 ± 0.3)

0.17 ± 0.00 (0.09 ± 0.00)

M. ordensis BRIP 26904 a

0.04 ± 0.00 (0.04 ± 0.00)

0.30 ± 0.01 (0.62 ± 0.01)

0.0 ± 0.0 (0.0 ± 0.0)

0.00 ± 0.00 (0.00 ± 0.00)

30.6 ± 2.3 (79.6 ± 2.5)

0.08 ± 0.01 (0.21 ± 0.01)

U. xerochloae BRIP 60876 a

0.04 ± 0.00 (0.04 ± 0.00)

0.26 ± 0.01 (0.65 ± 0.00)

0.0 ± 0.0 (0.0 ± 0.0)

0.00 ± 0.00 (0.00 ± 0.00)

26.8 ± 1.4 (37.3 ± 2.1)

0.07 ± 0.00 (0.10 ± 0.01)

S. iseilematis-ciliati BRIP 60887 a

0.04 ± 0.00 (0.04 ± 0.00)

0.38 ± 0.00 (0.50 ± 0.00)

8.5 ± 1.8 (0.0 ± 0.0)

0.02 ± 0.01 (0.00 ± 0.00)

16.4 ± 2.8 (1.4 ± 0.4)

0.04 ± 0.01 (0.00 ± 0.00)

  1. Comparison of growth rate, glycerol uptake rate and organic acid (itaconate, malate) titer and production rate before and after ALE. Values in brackets correlate to values after ALE. ±values indicate deviation from the mean (n = 2)