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Table 1 RAC incubated with MtLPMO9B only and with AbPPO or MtPPO7 addition in the presence of 21 reducing agents

From: Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Gr. Sub-group No. Reducing agent Activity [%] MtLPMO9Ba Activity [%] MtLPMO9B + AbPPOb Activity [%] MtLPMO9B + MtPPO7b
I a 1 4-Hydroxybenzoic acid 100 119 90
2 Ortho-cresol 100 778 2
3 Para-coumaric acid 100 9938 2851
4 Phenol 0c >0 c 0c
I b 5 3-Hydroxy-4-methoxycinnamic acid 100 151 7558
6 4-Hydroxy-3-methoxyphenylacetone 100 117 131
7 Coniferyl aldehyde 100 128 562
8 Ferulic acid 100 107 231
9 Guaiacol 100 461 5143
10 Hesperidin 100 56 771
11 Homovanillic acid 100 156 580
12 Vanillic acid 100 196 495
I c 13 4-Allyl-2,4-methoxyphenol 100 89 108
14 Sinapic acid 100 110 195
15 Syringic acid 100 107 142
II a 16 3,4-Dihydroxybenzoic acid 100 99 139
17 3-Methylcatechol 100 58 104
18 caffeic acid 100 55 122
II b 19 3,4-Dihydroxy-5-methoxybenzoic acid 100 99 22
20 3,4-Dihydroxy-5-methoxycinnamic acid 100 99 116
III   21 Gallic acid 100 105 124
  1. Division based on functional groups of reducing agents (Fig. 2)
  2. aTotal release of non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B set to 100%
  3. bIncreased (italics ≥50% increase) or decreased percentage of released non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B with addition of either AbPPO or MtPPO7 compared to the release of non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B alone. Sum of areas of released non-oxidized and C1-oxidized gluco-oligosaccharides are shown in Fig. 2. See “Methods” for more information
  4. cNo reference due to absent activity of MtLPMO9B towards RAC in the presence of phenol (Fig. 2)