Fig. 5
From: The significance of proline and glutamate on butanol chaotropic stress in Bacillus subtilis 168
Effect of exogenous addition of glutamate and proline on a growth-dependent butanol tolerance of B. subtilis 168. Cells were grown in SMM supplemented with 1.4% (vol/vol) butanol. Cell optical density was interval monitored to represent cell butanol tolerance. As a control, cells were grown in normal conditions without butanol addition (referred to as “None”) or in the presence of glutamate at 1 or 10 mM, or proline at 1 or 10 mM, each of which was exogenously added in the medium. Data are means of the results from at least three individual experiments. Error bars indicate standard errors