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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Recombinant expression of thermostable processive MtEG5 endoglucanase and its synergism with MtLPMO from Myceliophthora thermophila during the hydrolysis of lignocellulosic substrates

Fig. 1

a Time course of MtEG5A endoglucanase activity (gray bar) and biomass (black circle) production of the recombinant P. pastoris harboring the eg5a gene. The endoglucanase was expressed in culture broth by induction with 0.5% methanol and measured with β-glucan as substrate. b SDS-PAGE of MtEG5A during fermentation. Lane 1 Novex® sharp pre-stained protein marker, lanes 2–5 samples from fermentation culture broth at 99, 118, 130, and 153 h of incubation, lane 6 culture broth with MtEG5A after ultrafiltration

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