Fig. 7
Characterization of central metabolic model in developing L. glauca fruits for the regulation of carbon partitioning and energy supply for oil synthesis. The identified metabolic routes of carbon flux allocation and energy provision for FA synthesis are based on the transcriptome data from Illumina and 454 sequencing, and temporal transcript pattern analysis by qRT-PCR. Purple arrows indicate carbon flux drains into oil synthesis, brown arrows represent the transports of metabolite and energy across intracellular membrane by specific transporters, and green curved arrows represent all reactions for generation and consumption of energy in FA biosynthetic pathway, oxidative pentose phosphate pathway (OPPP), glycolysis, and tricarboxylic acid (TCA) cycle. All transporters and enzymes involved in carbon flux allocation and energy provision for FA synthesis are shown in red. Abbreviations for the enzymes, metabolites, and transporters are as follows: AAC ATP/ADP carrier, ACC acetyl-CoA carboxylase, ACLB ATP-citrate lyase subunit B, ACO aconitate hydratase, ADNT adenine nucleotide carrier, CS citrate synthase, DGAT diacylglycerol (DAG) acyltransferase, DIC dicarboxylate carrier, DTC dicarboxylate/tricarboxylate carrier, EAR enoyl-ACP reductase, ENO enolase, ER endoplasmic reticulum, FA fatty acid, FAD FA desaturase, FATA fatty acyl-ACP thioesterase A, FATB fatty acyl-ACP thioesterase B, FBA fructose-bisphosphate (F1,6P) aldolase, FK fructokinase, FUM fumarase, G6PDH glucose-6-phosphate (G6P) dehydrogenase, GAPA/C glyceraldehyde 3-phosphate (GAP) dehydrogenase subunit A/C, GLT glycolipid transporter, GPAT G3P acyltransferase, GPI G6P isomerase, GPT G6P transporter, HXK hexokinase, IDH isocitrate dehydrogenase, INV invertase, KAR ketoacyl-ACP reductase, LACS long-chain acyl-CoA synthase, LPAAT lysoPA acyltransferases, LPCAT lysoPC acyltransferases, LSC succinyl-CoA synthetase, MDH malate (Mal) dehydrogenase, ME malic enzyme, NTT nucleoside triphosphate (NTP) transporter, OGDHC 2-oxoglutarate dehydrogenase, 6PGDH 6-phosphogluconate (6PG) dehydrogenase, PA phosphatidic acid, PAP PA phosphohydrolases, PC phosphatidylcholine, PDAT DAG acyltransferase, PDC pyruvate (PYR) dehydrogenase complex, PEPC phosphoenolpyruvate (PEP) carboxylase, PFK ATP-dependent phosphofructokinase, PFP pyrophosphate phosphofructokinase, PGK phosphoglycerate kinase, PGLS 6-phosphogluconolactonase, PGM phosphoglycerate mutase, PHT phosphate (Pi) transporter, PK PYR kinase, PPT PEP transporter, PRK phosphoribulokinase, RBCS ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit, RPE ribulose-5-phosphate (Ru5P) epimerase, RPI ribose 5-phosphate isomerase, SDH succinate dehydrogenase, SUC sucrose transporter, SUS sucrose synthase, TA transaldolase, TAG triacylglycerol, TK transketolase, TPI triose phosphate isomerase, TPT triose phosphate transporters, UGP UDP-glucose (UDPG) pyrophosphorylase