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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Genome-wide characterization of cellulases from the hemi-biotrophic plant pathogen, Bipolaris sorokiniana, reveals the presence of a highly stable GH7 endoglucanase

Fig. 3

Biochemical characterization of BsGH7-3. a 10% SDS-PAGE of purified BsGH7-3. Lane M PageRuler plus pre-stained protein ladder (ThermoFisher, Waltham, USA) and BsGH7-3 stained by Coomassie Brilliant Blue R250. b Specific activity and stability of BsGH7-3 reported as percent residual activity at 4 and 60 °C, respectively. c Effect of temperature on GH7-3 measured over a range of 48 to 68 °C with 0.8% CMC as a substrate and McIlvaine buffer, pH 8.1. The residual activity was measured by the standard DNS assay and reported as % specific activity. d Effect of pH was determined by incubating the enzyme in buffer of pH range 5.2–8.6 for 6 h at 4 °C and then the kinetic assay was performed to determine the % specific activity

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