Fig. 5

Comparative analysis of B. sorokiniana GH7-3 with H. insolens endoglucanase GH7 (PDB ID: 1OJJ-B) and T. reesei cellobiohydrolase GH7 (PDB ID: 7CEL). (a) Multiple sequence alignment of BsGH7-3, HiGH7 and TrGH7. The black rectangular box contains a stretch of six residues of the catalytic motif that are conserved across the three proteins. The catalytic residues are marked with red arrows. The coloured rectangular boxes denote the residues of motifs B (green), A (red) and tunnel T (light yellow). b Homology model-based structure of BsGH7-3 shows variation in the secondary structure of motifs A, B and T (same colour as in a, with HiGH7 and TrGH7). The motifs A (red colour) and B (green colour) are in cartoon view on the mesh background. The surface view of the inner lining of the tunnel is shown in salmon colour