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Table 1 Effect of metal ions, salts, ionic liquids and detergents on the specific activity of BsGH7-3 and measured by standard spectrophotometric assay

From: Genome-wide characterization of cellulases from the hemi-biotrophic plant pathogen, Bipolaris sorokiniana, reveals the presence of a highly stable GH7 endoglucanase

Reagents Specific activity (%)
10 mM metal ions
 Magnesium chloride 91 ± 7
 Copper sulphate 100 ± 1
 Manganese chloride 444 ± 3
 Ferrous sulphate 304 ± 2
 Manganese chloride + ferrous sulphate 512 ± 5
 Ferric chloride 179 ± 1
 Calcium chloride 113 ± 5
 Nickel sulphate 114 ± 4
 Zinc acetate 97 ± 2
4000 mM salt
 Potassium chloride 124 ± 3
 Sodium chloride 109 ± 2
20% ionic liquid
 [C2C1im][C2C2PO4] 112 ± 3
 [C2C1im][MeCO2] 122 ± 2
 [C2C1im][Cl] 107 ± 3
7 mg mL−1 detergents
 Ariel™ 62 ± 5
 Tide™ 69 ± 4
 Sunlight™ 74 ± 4
 SDS 51 ± 3
  1. 100% specific activity = GH7-3 CMC activity in the absence of any additives